Gürkan Ali, Becerik Sema, Öztürk Veli Özgen, Atmaca Harika, Atilla Gül, Emingil Gülnur
Department of Periodontology, School of Dentistry, Ege University, İzmir, Turkey.
Department of Periodontology, School of Dentistry, Adnan Menderes University, Aydın, Turkey.
J Periodontol. 2015 Sep;86(9):1069-77. doi: 10.1902/jop.2015.150003. Epub 2015 Apr 30.
Interleukin (IL)-6 family of cytokines, including IL-6, oncostatin M (OSM), leukemia inhibitory factor (LIF), and IL-11, have fibrogenic features. The current study determines gingival crevicular fluid (GCF) levels of fibrosis-related IL-6-type cytokines in cyclosporine A (CsA)-induced gingival overgrowth (GO).
Eighty non-smokers were included (40 CsA-medicated renal transplant patients with GO [GO+; n = 20] or without GO [GO-; n = 20], 20 individuals with gingivitis, and 20 healthy participants). Probing depth and plaque, papilla bleeding, and hyperplastic index scores were recorded. GCF samples were obtained from the mesio-buccal aspects of two teeth. GCF IL-6, IL-1β, OSM, LIF, and IL-11 levels were analyzed by enzyme-linked immunosorbent assay.
The GO+ and GO- groups had higher IL-6 total amounts than the healthy group (P <0.008). IL-1β total amounts in the GO+ group were significantly higher than in both the healthy and GO- groups (P <0.008). OSM total amount was elevated in the GO+ and GO- groups compared with both the gingivitis and healthy groups (P <0.008). All groups had similar LIF and IL-11 total amounts (P >0.008). Moderate positive correlations were detected among IL-6, IL-1β, OSM, and IL-11 total amount in GCF and clinical parameters (P <0.05).
IL-6 and OSM increases in GCF as a result of CsA usage or an immunosuppressed state irrespective of the severity of inflammation and the presence of GO. The IL-6 family of cytokines might not be directly involved in biologic mechanisms associated with CsA-induced GO. Lack of an association between assessed IL-6 cytokines and CsA-induced GO might indicate distinct effects of these cytokines on fibrotic changes of different tissues.
白细胞介素（IL）-6细胞因子家族，包括IL-6、抑瘤素M（OSM）、白血病抑制因子（LIF）和IL-11，具有促纤维化特性。本研究测定环孢素A（CsA）诱导的牙龈过度生长（GO）中纤维化相关IL-6型细胞因子的龈沟液（GCF）水平。
纳入80名不吸烟者（40名接受CsA治疗的肾移植患者，其中有GO者[GO+；n = 20]或无GO者[GO-；n = 20]，20名牙龈炎患者，以及20名健康参与者）。记录探诊深度、菌斑、龈乳头出血和增生指数评分。从两颗牙齿的近中颊侧获取GCF样本。采用酶联免疫吸附测定法分析GCF中IL-6、IL-1β、OSM、LIF和IL-11水平。
GO+组和GO-组的IL-6总量高于健康组（P <0.008）。GO+组的IL-1β总量显著高于健康组和GO-组（P <0.008）。与牙龈炎组和健康组相比，GO+组和GO-组的OSM总量升高（P <0.008）。所有组的LIF和IL-11总量相似（P >0.008）。GCF中IL-6、IL-1β、OSM和IL-11总量与临床参数之间存在中度正相关（P <0.05）。
无论炎症严重程度和GO的存在与否，CsA的使用或免疫抑制状态都会导致GCF中IL-6和OSM增加。IL-6细胞因子家族可能不直接参与与CsA诱导的GO相关的生物学机制。评估的IL-6细胞因子与CsA诱导的GO之间缺乏关联可能表明这些细胞因子对不同组织纤维化变化的独特作用。
