Trauma Research Center, Baqiyatallah University of Medical Science, Tehran, Iran.
Int J Surg. 2012;10(9):537-41. doi: 10.1016/j.ijsu.2012.08.009. Epub 2012 Aug 27.
Adhesion formation after abdominal surgery is a major cause of postoperative bowel obstruction, infertility, and chronic abdominal pain. In this study, we evaluated the effect of normal saline and methylene blue (MB) on postoperative adhesion formation in a rat cecum model.
A total of 30 Wistar female rats in 2 treatment and 1 control groups underwent midline laparotomy and standardized abrasion of the visceral peritoneum. Normal saline and methylene blue were administrated intraperitoneally at the end of the surgical procedure in 2 treatment groups. Fourteen days after surgery, a re-laparotomy was performed for macroscopic and pathological assessment.
The adhesion grade and extent of the normal saline group was lower than control and MB groups in macroscopic assessment (P<0.05 for both). A comparison of adhesion stages in pathological assessment showed increment in abdominal adhesion by usage methylene blue 1% and demonstrated significant difference between MB and 2 other groups (P<0.05).
Administrated normal saline individually reduce the adhesion grade near cecum. Conversely, usage of methylene blue 1% may unpredictably increase risk of adhesion formation.
腹部手术后粘连形成是术后肠梗阻、不孕和慢性腹痛的主要原因。在这项研究中，我们评估了生理盐水和亚甲蓝（MB）对大鼠盲肠模型术后粘连形成的影响。
2 个治疗组和 1 个对照组共 30 只 Wistar 雌性大鼠接受了中线剖腹术，并对内脏腹膜进行了标准化的磨损。在手术结束时，2 个治疗组分别向腹腔内给予生理盐水和亚甲蓝。术后 14 天，进行再次剖腹术进行宏观和病理评估。
在宏观评估中，生理盐水组的粘连等级和程度均低于对照组和 MB 组（均 P<0.05）。在病理评估中比较粘连阶段显示，使用 1%亚甲蓝会增加腹部粘连，且与其他 2 组相比差异有统计学意义（P<0.05）。
单独使用生理盐水可降低盲肠附近的粘连等级。相反，使用 1%亚甲蓝可能会不可预测地增加粘连形成的风险。
