肺炎衣原体诱导大鼠肺癌的实验模型。
An experimental model for induction of lung cancer in rats by Chlamydia pneumoniae.
作者信息
Chu De-Jie, Guo Shui-Gen, Pan Chun-Feng, Wang Jing, Du Yong, Lu Xu-Feng, Yu Zhu-Yuan
机构信息
Department of Pulmonary Medicine, Jinshan Hospital, Fudan University, Shanghai, China.
出版信息
Asian Pac J Cancer Prev. 2012;13(6):2819-22. doi: 10.7314/apjcp.2012.13.6.2819.
OBJECTIVE
To assess induction effects of Chlamydia pneumoniae (Cpn) on lung cancer in rats.
METHODS
A lung cancer animal model was developed through repeated intratracheal injection of Cpn (TW-183) into the lungs of rats, with or without exposure to benzo(a)pyrene (Bp). Cpn antibodies (Cpn-IgA, -IgG, and -IgM) in serum were measured by microimmunofluorescence. Cpn-DNA or Cpn-Ag of rat lung cancer was detected through polymerase chain reaction or enzyme-linked immunosorbent assay.
RESULTS
The prevalence of Cpn infection was 72.9% (35/48) in the Cpn group and 76.7% (33/43) in the Cpn plus benzo(a)pyrene (Bp) group, with incidences of lung carcinomas in the two groups of 14.6% (7/48) and 44.2% (19/43), respectively (P-values 0.001 and <0.001 compared with normal controls).
CONCLUSIONS
A rat model of lung carcinoma induced by Cpn infection was successfully established in the laboratory for future studies on the treatment, prevention, and mechanisms of the disease.
目的
评估肺炎衣原体（Cpn）对大鼠肺癌的诱导作用。
方法
通过向大鼠肺部反复气管内注射Cpn（TW-183），无论是否暴露于苯并(a)芘（Bp），建立肺癌动物模型。采用微量免疫荧光法检测血清中的Cpn抗体（Cpn-IgA、-IgG和-IgM）。通过聚合酶链反应或酶联免疫吸附测定法检测大鼠肺癌组织中的Cpn-DNA或Cpn-Ag。
结果
Cpn组的Cpn感染率为72.9%（35/48），Cpn加苯并(a)芘（Bp）组为76.7%（33/43），两组肺癌发生率分别为14.6%（7/48）和44.2%（19/43）（与正常对照组相比，P值分别为0.001和<0.001）。
结论
在实验室成功建立了Cpn感染诱导的大鼠肺癌模型，以供今后对该疾病的治疗、预防和发病机制进行研究。
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