Chu De-Jie, Guo Shui-Gen, Pan Chun-Feng, Wang Jing, Du Yong, Lu Xu-Feng, Yu Zhu-Yuan
Department of Pulmonary Medicine, Jinshan Hospital, Fudan University, Shanghai, China.
Asian Pac J Cancer Prev. 2012;13(6):2819-22. doi: 10.7314/apjcp.2012.13.6.2819.
To assess induction effects of Chlamydia pneumoniae (Cpn) on lung cancer in rats.
A lung cancer animal model was developed through repeated intratracheal injection of Cpn (TW-183) into the lungs of rats, with or without exposure to benzo(a)pyrene (Bp). Cpn antibodies (Cpn-IgA, -IgG, and -IgM) in serum were measured by microimmunofluorescence. Cpn-DNA or Cpn-Ag of rat lung cancer was detected through polymerase chain reaction or enzyme-linked immunosorbent assay.
The prevalence of Cpn infection was 72.9% (35/48) in the Cpn group and 76.7% (33/43) in the Cpn plus benzo(a)pyrene (Bp) group, with incidences of lung carcinomas in the two groups of 14.6% (7/48) and 44.2% (19/43), respectively (P-values 0.001 and <0.001 compared with normal controls).
A rat model of lung carcinoma induced by Cpn infection was successfully established in the laboratory for future studies on the treatment, prevention, and mechanisms of the disease.
评估肺炎衣原体（Cpn）对大鼠肺癌的诱导作用。
通过向大鼠肺部反复气管内注射Cpn（TW-183），无论是否暴露于苯并(a)芘（Bp），建立肺癌动物模型。采用微量免疫荧光法检测血清中的Cpn抗体（Cpn-IgA、-IgG和-IgM）。通过聚合酶链反应或酶联免疫吸附测定法检测大鼠肺癌组织中的Cpn-DNA或Cpn-Ag。
Cpn组的Cpn感染率为72.9%（35/48），Cpn加苯并(a)芘（Bp）组为76.7%（33/43），两组肺癌发生率分别为14.6%（7/48）和44.2%（19/43）（与正常对照组相比，P值分别为0.001和<0.001）。
在实验室成功建立了Cpn感染诱导的大鼠肺癌模型，以供今后对该疾病的治疗、预防和发病机制进行研究。
