[肺癌患者肺炎衣原体IgG阳性与放射性肺损伤相关细胞因子的相关性]

[Correlation between chlamydia pneumoniae IgG positive in lung cancer patients and cytokines related to radiation-induced pulmonary lesion].

作者信息

Zhang Wenyi, Qiao Tiankui, Zhou Daoan, Yuan Sujuan

机构信息

Department of Radiation Oncology, Jinshan Hospital, Fudan University, Shanghai 200540, China.

出版信息

Zhongguo Fei Ai Za Zhi. 2011 Feb;14(2):132-6. doi: 10.3779/j.issn.1009-3419.2011.02.05.

DOI:10.3779/j.issn.1009-3419.2011.02.05

PMID:21342643

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5999776/

Abstract

BACKGROUND AND OBJECTIVE

There exists intimate relationship between infection with chlamydia pneumoniae (Cpn) and lung cancer incidence. But few studies have been reported about radiation-induced pulmonary lesion in lung cancer patients infected with Cpn. The aim of this study is to explore the correlation between cytokines related to radiation-induced pulmonary lesion and Cpn IgG positive in lung cancer patients.

METHODS

A total of 69 patients with lung cancer received chest radiotherapy. Blood samples were collected and frozen before radiotherapy (pre-RT), middle radiotherapy (mid-RT) and after radiotherapy (post-RT). Cpn IgG and levels of IL-1β, SP-A, TGF-β, and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).

RESULTS

In the total of 69 patients, 21 patients were Cpn IgG positive, 48 patients negative. The positive rate was 30.43%. In mid-RT concentration of IL-1β in Cpn IgG positive and negative group were (35.82±10.09) ng/L and (30.01±6.46) ng/L, with statistically significant difference (P < 0.05). Pre-RT and post-RT concentrations of IL-1β in Cpn IgG positive and negative group had no statistically significant difference. Mid-RT concentrations of SP-A in Cpn IgG positive group and negative group were (641.78±106.81) ng/L and (100.86±61.4) ng/L respectively, with statistically significant difference (P < 0.05). Post-RT concentration of SP-A in Cpn IgG positive and negative group were (657.47±115.19) ng/L and (93.23±47.15) ng/L respectively, with statistically significant difference (P < 0.05). Concentrations of TNF-α in Cpn IgG positive and negative group had no statistically significant difference. Concentrations of TGF-β in Cpn IgG positive group were (710.67±358.16) pg/mL in pre-RT, (1,002.06±542.16) pg/mL in mid-RT, (2,125.16±1,522.29) pg/mL in post-RT; those in negative group were (867.77±412.48) pg/mL, (914.05±425.70) pg/mL, (1,073.36±896.01) pg/mL. Concentration of TGF-β in post-RT between Cpn IgG positive and negative group had statistically significant difference (P < 0.05).

CONCLUSIONS

Cpn IgG positive in lung cancer patients influenced levels of IL-1β, SP-A, TGF-β during chest radiotherapy. This might aggravate radiation-induced pulmonary lesion.

摘要

背景与目的

肺炎衣原体（Cpn）感染与肺癌发病之间存在密切关系。但关于Cpn感染的肺癌患者放射性肺损伤的研究报道较少。本研究旨在探讨肺癌患者放射性肺损伤相关细胞因子与Cpn IgG阳性之间的相关性。

方法

69例肺癌患者接受胸部放疗。在放疗前（RT前）、放疗中期（RT中期）和放疗后（RT后）采集血样并冷冻保存。采用酶联免疫吸附测定（ELISA）法检测Cpn IgG以及白细胞介素-1β（IL-1β）、表面活性蛋白A（SP-A）、转化生长因子-β（TGF-β）和肿瘤坏死因子-α（TNF-α）的水平。

结果

69例患者中，21例Cpn IgG阳性，48例阴性。阳性率为30.43%。在RT中期，Cpn IgG阳性组和阴性组的IL-1β浓度分别为（35.82±10.09）ng/L和（30.01±6.46）ng/L，差异有统计学意义（P<0.05）。Cpn IgG阳性组和阴性组在RT前和RT后的IL-1β浓度差异无统计学意义。RT中期，Cpn IgG阳性组和阴性组的SP-A浓度分别为（641.78±106.81）ng/L和（100.86±61.4）ng/L，差异有统计学意义（P<0.05）。RT后，Cpn IgG阳性组和阴性组的SP-A浓度分别为（657.47±115.19）ng/L和（93.23±47.15）ng/L，差异有统计学意义（P<0.05）。Cpn IgG阳性组和阴性组的TNF-α浓度差异无统计学意义。Cpn IgG阳性组放疗前、放疗中期、放疗后的TGF-β浓度分别为（710.67±358.16）pg/mL、（1002.06±542.16）pg/mL、（2125.16±1522.29）pg/mL；阴性组分别为（867.77±412.48）pg/mL、（914.05±425.70）pg/mL、（1073.36±896.）pg/mL。Cpn IgG阳性组和阴性组放疗后的TGF-β浓度差异有统计学意义（P<0.05）。