Université de Perpignan via Domitia, IMAGES, France.
Anal Chim Acta. 2012 Oct 1;745:64-9. doi: 10.1016/j.aca.2012.07.018. Epub 2012 Aug 8.
Among known pesticide groups, organophosphates (OPs) have grasped attention due to their hazardous nature and their applications as pesticides and chemical weapons. This work presents the development of cost-effective column based biosensor for detoxification of OPs in water and milk. Enzyme phosphotriesterase (PTE) was immobilized on an activated Sepharose 4B via covalent coupling using an Omnifit glass column. Three different OPs, ethyl paraoxon (EPOx), malaoxon (MAO) and chlorpyriphos-oxon (CPO) were spiked in water and milk to test the detoxification of OPs. Mixtures of these pesticides were also tested to check the cumulative detoxification in the real samples. The efficiency of detoxification was evaluated using a highly sensitive acetylcholinesterase (AChE) B394 biosensor based flow system. The column conditions were optimized for the detoxification studied. The method was shown to be promising when we tested real milk samples spiked with OPs. Detoxification obtained in milk was up to 86% whereas in water, 100% detoxification was obtained.
在已知的农药类别中，有机磷化合物（OPs）因其危害性以及作为农药和化学武器的应用而备受关注。本工作提出了一种经济高效的基于柱的生物传感器，用于水和牛奶中有机磷化合物的解毒。酶磷酸三酯酶（PTE）通过使用 Omnifit 玻璃柱的共价偶联固定在活化的 Sepharose 4B 上。将三种不同的有机磷化合物，乙基对氧磷（EPOx）、马拉氧磷（MAO）和毒死蜱氧磷（CPO）加入到水中和牛奶中，以测试有机磷化合物的解毒情况。还测试了这些杀虫剂的混合物，以检查实际样品中的累积解毒情况。使用基于高度敏感的乙酰胆碱酯酶（AChE）B394 的生物传感器的流动系统评估了解毒效率。对所研究的解毒进行了柱条件的优化。当我们测试加入有机磷化合物的实际牛奶样品时，该方法显示出了很大的应用前景。在牛奶中获得的解毒率高达 86%，而在水中，100%的解毒率。
