Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
Biosens Bioelectron. 2013 Jan 15;39(1):320-3. doi: 10.1016/j.bios.2012.07.026. Epub 2012 Jul 21.
We developed a highly sensitive flow injection/amperometric biosensor for the detection of organophosphate pesticides (OPs) using layered double hydroxides (LDHs) as the immobilization matrix of acetylcholinesterase (AChE). LDHs provided a biocompatible microenvironment to keep the bioactivity of AChE, due to the intrinsic properties of LDHs (such as a regular structure, good mechanical, chemical and thermal stabilities, and swelling properties). By integrating the flow injection analysis (FIA) with amperometric detection, the resulting AChE-LDHs modified electrode greatly catalyzed the oxidation of the enzymatically generated thiocholine product, and facilitated the detection automation, thus increasing the detection sensitivity. The analytical conditions for the FIA/amperometric detection of OPs were optimized by using methyl parathion (MP) as a model. The inhibition of MP was proportional to its concentration ranging from 0.005 to 0.3μg mL(-1) and 0.3 to 4.0μg mL(-1) with a detection limit 0.6ng mL(-1) (S/N=3). The developed biosensor exhibited good reproducibility and acceptable stability.
我们开发了一种基于层状双氢氧化物（LDHs）作为乙酰胆碱酯酶（AChE）固定基质的高灵敏度流动注射/安培生物传感器，用于检测有机磷农药（OPs）。LDHs 提供了一个生物相容性的微环境，以保持 AChE 的生物活性，这是由于 LDHs 的固有特性（如规则的结构、良好的机械、化学和热稳定性以及溶胀性能）。通过将流动注射分析（FIA）与安培检测相结合，所得的 AChE-LDHs 修饰电极极大地催化了酶促生成的硫代胆碱产物的氧化，促进了检测自动化，从而提高了检测灵敏度。采用甲基对硫磷（MP）作为模型，优化了 FIA/安培检测 OPs 的分析条件。MP 的抑制与浓度成正比，范围为 0.005 至 0.3μg mL（-1）和 0.3 至 4.0μg mL（-1），检测限为 0.6ng mL（-1）（S/N=3）。所开发的生物传感器表现出良好的重现性和可接受的稳定性。
