Plant Ecology and Environment Science Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, UP, India.
J Hazard Mater. 2013 Nov 15;262:1039-47. doi: 10.1016/j.jhazmat.2012.08.019. Epub 2012 Aug 17.
An arsenic hypertolerant bacterium was isolated from arsenic contaminated site of West Bengal, India. The bacteria was identified as Staphylococcus arlettae strain NBRIEAG-6, based on 16S rDNA analysis. S. arlettae was able to remove arsenic from liquid media and possesses arsC gene, gene responsible for arsenate reductase activity. The biochemical profiling of the isolated strain showed that it had the capacity of producing indole acetic acid (IAA), siderophores and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Furthermore, an experiment was conducted to test the effect of S. arlettae inoculation on concurrent plant growth promotion and arsenic uptake in Indian mustard plant [Brassica juncea (L.) Czern. Var. R-46] when grown in arsenic spiked (5, 10 and 15 mg kg(-1)) soil. The microbial inoculation significantly (p<0.05) increased biomass, protein, chlorophyll and carotenoids contents in test plant. Moreover, as compared to the non-inoculated control, the As concentration in shoot and root of inoculated plants were increased from 3.73 to 34.16% and 87.35 to 99.93%, respectively. The experimental results show that the plant growth promoting bacteria NBRIEAG-6 has the ability to help B. juncea to accumulate As maximally in plant root, and therefore it can be accounted as a new bacteria for As phytostabilization.
从印度西孟加拉邦砷污染场地分离到一株砷耐受菌。根据 16S rDNA 分析，该菌被鉴定为葡萄球菌属的 NBRIEAG-6 菌株。葡萄球菌属能够从液体培养基中去除砷，并具有 arsC 基因，该基因负责砷酸盐还原酶活性。分离株的生化特征分析表明，它具有产生吲哚乙酸（IAA）、铁载体和 1-氨基环丙烷-1-羧酸（ACC）脱氨酶的能力。此外，进行了一项实验，以测试当在砷污染（5、10 和 15 mg kg(-1))土壤中种植印度芥菜[Brassica juncea (L.) Czern. Var. R-46]时，接种葡萄球菌属对植物生长促进和砷吸收的影响。微生物接种显著（p<0.05）增加了试验植物的生物量、蛋白质、叶绿素和类胡萝卜素含量。此外，与未接种对照相比，接种植物的地上部分和根部的砷浓度分别从 3.73%增加到 34.16%和从 87.35%增加到 99.93%。实验结果表明，植物促生菌 NBRIEAG-6 具有帮助印度芥菜在植物根部最大限度地积累砷的能力，因此可以将其视为一种新的砷植物稳定化细菌。
