人牙周膜成纤维细胞对釉基质衍生物的摄取和处理。
Cellular uptake and processing of enamel matrix derivative by human periodontal ligament fibroblasts.
机构信息
Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, England, United Kingdom.
出版信息
Arch Oral Biol. 2013 Apr;58(4):348-54. doi: 10.1016/j.archoralbio.2012.08.003. Epub 2012 Aug 29.
OBJECTIVE
Enamel matrix derivative (EMD), is an extract of porcine developing enamel matrix. Its commercialised form Emdogain, is claimed to stimulate periodontal regeneration by recapitulating original developmental processes, although the mechanism remains unclear. Our objective was to investigate interactions between EMD and human periodontal ligament (HPDL) fibroblasts in vitro.
DESIGN
HPDL fibroblasts were cultured in the presence of fluorescently labelled EMD and cellular EMD uptake was monitored using confocal laser scanning microscopy and immunohistochemistry. Internalised EMD proteins were characterised using SDS-PAGE.
RESULTS
EMD was internalised by HPDL fibroblasts leading to the appearance of multiple, vesicle-like structure in the cytoplasm. The internalised protein was composed mainly of the major 20kDa amelogenin component of EMD which was subsequently processed with time to generate a cumulative 5kDa component.
CONCLUSIONS
Cellular uptake and subsequent intracellular processing of EMD components by dental mesenchymal cells may play a role in EMD bioactivity and in part explain the turnover of Emdogain when placed clinically.
目的
釉基质衍生物（EMD）是猪发育中釉质基质的提取物。其商业化形式 Emdogain 据称通过再现原始发育过程来刺激牙周组织再生，尽管其机制尚不清楚。我们的目的是研究 EMD 与人类牙周韧带（HPDL）成纤维细胞在体外的相互作用。
设计
将 HPDL 成纤维细胞在荧光标记的 EMD 存在下培养，并使用共焦激光扫描显微镜和免疫组织化学监测细胞内 EMD 摄取。使用 SDS-PAGE 对内化的 EMD 蛋白进行表征。
结果
EMD 被 HPDL 成纤维细胞内化，导致细胞质中出现多个囊泡样结构。内化的蛋白主要由 EMD 的主要 20kDa 釉原蛋白组成，随着时间的推移，该蛋白被进一步加工生成累积的 5kDa 成分。
结论
牙间质细胞对 EMD 成分的细胞摄取和随后的细胞内加工可能在 EMD 的生物活性中起作用，并部分解释了临床上放置 Emdogain 时的周转。
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