Faculty of Odontology, University Paul-Sabatier, 3 Chemin des Maraîchers, 31062 Toulouse Cedex, France.
Biomaterials. 2011 Oct;32(30):7375-88. doi: 10.1016/j.biomaterials.2011.06.043. Epub 2011 Jul 23.
Periodontitis is a chronic-, infectious-disease of the human periodontium that is characterized by the loss of supporting tissues surrounding the tooth such as the periodontal ligament (PDL), cementum and alveolar bone. Regeneration of the periodontium is dependent on the participation of mesenchymal stem/stromal cells (MSC) resident in the PDL. Enamel matrix derivative (EMD), an extract from immature porcine enamel rich in amelogenin protein but that also contain bone morphogenetic protein (BMP), is used to treat periodontal defects. The effects of EMD on MSC cells of the PDL are not well characterized. In this in vitro study, we identify PDL progenitor cells from multiple individuals and demonstrate that EMD stimulates them. We show that the effect of EMD on cell proliferation and migration is mediated through the amelogenin it contains, while the differentiation of these progenitor cells to cell types of mineralized tissue is mainly due to BMP signaling.
牙周炎是一种慢性、感染性的人类牙周疾病，其特征是牙齿周围的支持组织如牙周韧带（PDL）、牙骨质和牙槽骨的丧失。牙周组织的再生依赖于驻留在牙周韧带中的间充质干细胞/基质细胞（MSC）的参与。釉基质衍生物（EMD）是一种从富含釉原蛋白的未成熟猪牙釉质中提取的物质，但也含有骨形态发生蛋白（BMP），用于治疗牙周缺损。EMD 对 PDL 间充质干细胞的作用尚未得到很好的描述。在这项体外研究中，我们从多个个体中鉴定出 PDL 祖细胞，并证明 EMD 能刺激它们。我们表明，EMD 对细胞增殖和迁移的影响是通过其所含的釉原蛋白介导的，而这些祖细胞向矿化组织细胞类型的分化主要归因于 BMP 信号。
