Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China.
Acta Trop. 2012 Dec;124(3):199-202. doi: 10.1016/j.actatropica.2012.08.006. Epub 2012 Aug 20.
Schistosomiasis remains a serious worldwide public health problem. Improving the diagnostic assay for surveillance and monitoring will contribute to hastening the possible elimination of the disease in endemic regions. Therefore, this study aims to develop magnetic affinity enzyme-linked immunoassay (MEIA) for serological diagnosis of schistosomiasis based on recombinant 26kDa glutathione-S-transferase of Schistosoma japonicum (rSj26GST). BALB/c mice infected with S. japonicum cercariae (40 per mouse) were used. After infecting for 6 weeks, the antibody was detected by MEIA. All of the infected mouse sera were effectively determined by MEIA. Compared with the enzyme-linked immunosorbent assay (ELISA), MEIA has a higher ratio of the mean positive value to the mean negative value (P/N) at the same dilution ratio (3.92 versus 2.66). MEIA was further applied for diagnosis of human schistosomiasis. Sera from 28 schistosomiasis-confirmed patients with low-intensity infection, 15 treated patients, and 20 non-endemic negative controls, were used to assess the assay. The results showed that MEIA and ELISA had similarity in positive detection rates. However, the higher P/N of MEIA was observed at the same dilution ratio. MEIA had high negative rate in detection of specific IgG in the treated patients. Moreover, there was no cross reaction with the sera of paragonimiasis patients. These results suggested that MEIA based on rSj26GST is a simple, rapid, convenient assay for the diagnosis of schistosomiasis.
日本血吸虫病仍然是一个严重的全球公共卫生问题。改善监测和监测的诊断检测方法将有助于加速在流行地区消除该疾病。因此，本研究旨在基于重组日本血吸虫 26kDa 谷胱甘肽-S-转移酶（rSj26GST）开发用于日本血吸虫病血清学诊断的磁性亲和酶联免疫吸附测定法（MEIA）。使用感染日本血吸虫尾蚴（每只 40 只）的 BALB/c 小鼠。感染 6 周后，通过 MEIA 检测抗体。所有感染的小鼠血清均通过 MEIA 有效确定。与酶联免疫吸附试验（ELISA）相比，在相同稀释比下，MEIA 的平均阳性值与平均阴性值的比值（P/N）更高（3.92 比 2.66）。MEIA 进一步应用于人类血吸虫病的诊断。使用 28 例低强度感染的血吸虫病确诊患者、15 例治疗患者和 20 例非流行地区阴性对照的血清来评估该检测方法。结果表明，MEIA 和 ELISA 在阳性检测率方面具有相似性。然而，在相同的稀释比下，MEIA 的 P/N 更高。MEIA 在检测治疗患者的特异性 IgG 时具有较高的阴性率。此外，与并殖吸虫病患者的血清无交叉反应。这些结果表明，基于 rSj26GST 的 MEIA 是一种用于诊断血吸虫病的简单、快速、方便的检测方法。
