School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, Bandar Sunway, 46150 Petaling Jaya, Selangor, Malaysia.
Bioresour Technol. 2012 Nov;123:290-5. doi: 10.1016/j.biortech.2012.07.082. Epub 2012 Jul 31.
Cu(II) removal efficacies of alginate-immobilized Trichoderma asperellum using viable and non-viable forms were investigated with respect to time, pH, and initial Cu(II) concentrations. The reusability potential of the biomass was determined based on sorption/desorption tests. Cu(II) biosorption by immobilized heat-inactivated T. asperellum cells was the most efficient, with 134.22mg Cu(II) removed g(-1) adsorbent, compared to immobilized viable cells and plain alginate beads (control) with 105.96 and 94.04mg Cu(II) adsorbed g(-1) adsorbent, respectively. Immobilized non-viable cells achieved equilibrium more rapidly within 4h. For all biosorbents, optimum pH for Cu(II) removal was between pH 4 and 5. Reusability of all biosorbents were similar, with more than 90% Cu(II) desorbed with HCl. These alginate-immobilized cells can be applied to reduce clogging and post-separation process incurred from use of suspended biomass.
研究了用活菌和死菌形式的海藻酸钠固定化aspergillus Trichoderma 去除 Cu(II)的效果，考察了时间、pH 值和初始 Cu(II)浓度的影响。根据吸附/解吸试验确定了生物质的可重复使用性。与固定化活菌和普通海藻酸钠珠（对照）相比，固定化热灭活 T. asperellum 细胞对 Cu(II)的吸附效率最高，吸附剂可去除 134.22mg Cu(II) g(-1)吸附剂，而固定化活菌和普通海藻酸钠珠（对照）分别可去除 105.96 和 94.04mg Cu(II) g(-1)吸附剂。固定化非活菌在 4 小时内更快达到平衡。对于所有的生物吸附剂，Cu(II)去除的最佳 pH 值在 4 到 5 之间。所有生物吸附剂的可重复使用性相似，用 HCl 可解吸超过 90%的 Cu(II)。这些海藻酸钠固定化细胞可用于减少悬浮生物量使用带来的堵塞和后续分离过程中的损失。
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