Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte, UFRN, Natal, RN, Brazil.
Food Chem Toxicol. 2012 Dec;50(12):4340-7. doi: 10.1016/j.fct.2012.08.003. Epub 2012 Aug 23.
The present study aimed to determine whether the leaves of Turnera ulmifolia Linn. var. elegans extract exert significant antioxidant activity. The antioxidant activity of its hydroethanolic extract (HEETU) was evaluated by assessing (a) its radical scavenging ability in vitro, and (b) its in vivo effect on lipid peroxidation and antioxidant enzyme activities. The in vitro antioxidant assay (DPPH) clearly supported HEETU free radical scavenging potential. Moreover, glutathione content and antioxidant enzyme activities (glutathione peroxidase, superoxide dismutase and catalase) were significantly enhanced in CCl(4)-treated rats due to oral HEETU-treatment (500 mg/kgb.w.) over 7 and 21 days. In addition, an improvement was observed in lipid peroxidation and serum biochemical parameters (aspartate aminotransferase and alanine aminotransferase), indicating a protective effect against CCl(4)-induced liver injuries, confirmed by histopathological studies. The HEETU effect was comparable to the standard drug Legalon® (50 mg/kgb.w.) under the same experimental condition. Quantitative analysis of the HPLC extract revealed the presence of flavonoids, wich mediate the effects of antioxidant and oxidative stress. In conclusion, extract components exhibit antioxidant and hepatoprotective activities in vitro and in vivo.
本研究旨在确定 Turnera ulmifolia Linn. var. elegans 叶提取物是否具有显著的抗氧化活性。通过评估其水乙醇提取物 (HEETU) 的（a）体外自由基清除能力和（b）体内对脂质过氧化和抗氧化酶活性的影响，来评估其抗氧化活性。体外抗氧化测定 (DPPH) 清楚地支持 HEETU 自由基清除能力。此外，由于口服 HEETU 处理（500mg/kgb.w.）7 天和 21 天，CCl(4)处理大鼠的谷胱甘肽含量和抗氧化酶活性（谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶）显著增强。此外，还观察到脂质过氧化和血清生化参数（天冬氨酸氨基转移酶和丙氨酸氨基转移酶）的改善，这表明对 CCl(4)诱导的肝损伤具有保护作用，通过组织病理学研究得到证实。在相同的实验条件下，HEETU 作用可与标准药物 Legalon®（50mg/kgb.w.）相媲美。HPLC 提取物的定量分析表明存在黄酮类化合物，介导抗氧化和氧化应激的作用。总之，提取物成分在体外和体内均表现出抗氧化和保肝活性。
