短短芽孢杆菌47中壁蛋白基因两个翻译起始位点的利用
Use of both translation initiation sites of the middle wall protein gene in Bacillus brevis 47.
作者信息
Adachi T, Yamagata H, Tsukagoshi N, Udaka S
机构信息
Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Japan.
出版信息
J Bacteriol. 1990 Jan;172(1):511-3. doi: 10.1128/jb.172.1.511-513.1990.
Abstract
The middle wall protein gene of Bacillus brevis 47 has two potential translation initiation sites located tandemly in the same reading frame. We demonstrate here that both sites are utilized to start translation in B. brevis 47. Translation from the first site (located upstream) gives rise to a precursor of the middle wall protein with an extension peptide of 31 amino acids preceding the signal peptide. The precursor was cleaved at the same position as that of the precursor translated from the second site. The TTG codon seems to play an appreciable role in the initiation of translation in B. brevis 47.
摘要
短短芽孢杆菌47的中壁蛋白基因在同一阅读框中有两个串联的潜在翻译起始位点。我们在此证明，在短短芽孢杆菌47中这两个位点均被用于起始翻译。从第一个位点（位于上游）起始的翻译产生了中壁蛋白的前体，在信号肽之前有一个31个氨基酸的延伸肽。该前体在与从第二个位点翻译的前体相同的位置被切割。TTG密码子似乎在短短芽孢杆菌47的翻译起始中起着重要作用。
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