构建用于生化和细胞生物学研究的含位点特异性DNA链间交联的质粒。
Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies.
作者信息
Enoiu Milica, Ho The Vinh, Long David T, Walter Johannes C, Schärer Orlando D
机构信息
Institute of Molecular Cancer Research, University of Zürich, Zürich, Switzerland.
出版信息
Methods Mol Biol. 2012;920:203-19. doi: 10.1007/978-1-61779-998-3_15.
Abstract
Plasmids containing a site-specific DNA interstrand cross-link (ICL) are invaluable tools for the investigation of ICL repair pathways at the biochemical and cellular level. We describe a procedure for preparation of plasmid DNA substrates containing a single ICL at a specific site. The procedure is versatile, leads to reliable yields of pure DNA substrate, and is suitable for the incorporation of virtually any type of DNA lesion into plasmids.
摘要
含有位点特异性DNA链间交联（ICL）的质粒是在生化和细胞水平研究ICL修复途径的宝贵工具。我们描述了一种制备在特定位点含有单个ICL的质粒DNA底物的方法。该方法用途广泛，能可靠地获得纯DNA底物，并且几乎适用于将任何类型的DNA损伤掺入质粒中。
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