Xu Lu-Hong, Fang Jian-Pei, Xu Hong-Gui, Weng Wen-Jun
Department of Pediatrics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Hematology. 2012 Jul;17(4):215-9. doi: 10.1179/102453312X13376952196539.
Sensitized recipients are at a high risk of graft rejection in hematopoietic stem cell transplantation. To explore the trace of donor cells, we tried to explore homing and engraftment of bone marrow cells (BMCs) derived from different donors in a murine model of sensitization. Sensitized BALB/c mice were used as transplanted recipients, which received BMCs derived from C57BL/6 or BALB/c donors after lethal irradiation. The homing study showed that the donor cells decreased along with time in recipients of the C57BL/6 donor group, but the donor cells increased along with time in recipients of the BALB/c donor group. For the engraftment assay, all the sensitized recipients transplanted with BMCs derived from C57BL/6 donors died after lethal irradiation. In contrast, all the recipients transplanted with BMCs derived from BALB/c donors got long-term survival. Our results suggest that it is crucial to have human leukocyte antigen identical donors for sensitized recipients during hematopoietic stem cell transplantation.
在造血干细胞移植中，致敏受体发生移植物排斥的风险很高。为了探索供体细胞的踪迹，我们试图在致敏小鼠模型中研究来自不同供体的骨髓细胞（BMC）的归巢和植入情况。将致敏的BALB/c小鼠作为移植受体，在致死性照射后接受来自C57BL/6或BALB/c供体的BMC。归巢研究表明，在C57BL/6供体组的受体中，供体细胞随时间减少，但在BALB/c供体组的受体中，供体细胞随时间增加。对于植入分析，所有接受来自C57BL/6供体的BMC移植的致敏受体在致死性照射后死亡。相比之下，所有接受来自BALB/c供体的BMC移植的受体都获得了长期存活。我们的结果表明，在造血干细胞移植期间，为致敏受体提供人类白细胞抗原相同的供体至关重要。
