Lü Zhi-dong, Xu Hui-mian, Wang Hai-bo, Kong Bin, Li Jian-guo, Li Fu-nian, Song Yong-mei
Department of Breast Surgery, Affiliated Hospital, Qingdao University Medical College, China.
Zhonghua Yi Xue Za Zhi. 2012 Jun 26;92(24):1698-701.
To elucidate the effect of transforming growth factor-beta 1 (TGF-β1) on peritoneal fibrosis and the regulation of gastric cancer adhering to mesothelial cells.
The peritoneal mesothelial cell line of HMrSV5 was used to determine the role of TGF-β1 in the regulation of gastric cancer cell adhering to mesothelial cells. And the mRNA and protein expressions of collagen III and fibronectin were detected by adhesion assay, Western blot, immunofluorescent staining and reverse transcription-polymerase chain reaction (RT-PCR).
(1) The treatment of 5 ng/ml TGF-β1 could induce the expressions of collagen III and fibronectin in mesothelial cells at 24, 48 and 72 h (P < 0.01). (2) As compared with the controls, the percentages of adhered HGC-27 and HSC-39 gastric cancer cells significantly increased under the treatment of TGF-β1 for 24 and 72 h. The increased adhesion percentages of HGC-27 were 65% ± 5% and 124% ± 11% (P < 0.05) while those of HSC-39 85% ± 9% and 146% ± 17% respectively (P < 0.05). (3) Arginyl-glycyl-aspartic acid (RGD) (knockdown of minimal sites for cell-binding domain of extracellular matrix) decreased the number of cancer cells adhering to mesothelial cells under the stimulation of TGF-β1. And the decreased adhesion percentage of HGC-27 was 65% ± 8% (P < 0.05).
TGF-β1 significantly stimulates the expressions of collagen III and fibronectin in mesothelial cells. And it is associated with the increased adhesion of gastric cancer cell and offers a favorable environment for the dissemination of gastric cancer.
阐明转化生长因子-β1（TGF-β1）对腹膜纤维化的影响以及对胃癌黏附于间皮细胞的调控作用。
采用人腹膜间皮细胞系HMrSV5，确定TGF-β1在调控胃癌细胞黏附于间皮细胞中的作用。通过黏附试验、蛋白质印迹法、免疫荧光染色及逆转录-聚合酶链反应（RT-PCR）检测Ⅲ型胶原和纤连蛋白的mRNA及蛋白表达。
（1）5 ng/ml TGF-β1处理24、48和72小时可诱导间皮细胞中Ⅲ型胶原和纤连蛋白的表达（P < 0.01）。（2）与对照组相比，TGF-β1处理24和72小时后，黏附的HGC-27和HSC-39胃癌细胞百分比显著增加。HGC-27增加的黏附百分比分别为65%±5%和124%±11%（P < 0.05），而HSC-39分别为85%±9%和146%±17%（P < 0.05）。（3）精氨酰-甘氨酰-天冬氨酸（RGD）（敲低细胞外基质细胞结合域的最小位点）在TGF-β1刺激下减少了黏附于间皮细胞的癌细胞数量。HGC-27减少的黏附百分比为65%±8%（P < 0.05）。
TGF-β1显著刺激间皮细胞中Ⅲ型胶原和纤连蛋白的表达。并且它与胃癌细胞黏附增加相关，为胃癌的播散提供了有利环境。
