Department of Obstetrics and Gynecology, University of Rostock, Suedring 81, Rostock 18059, Germany.
J Perinat Med. 2012 May 13;40(5):475-81. doi: 10.1515/jpm-2011-0277.
The expression of the anti-inflammatory glycoprotein progranulin and the hypoxia-induced transcription factor 1α (HIF-1α) in the villous trophoblast was compared between placentae from patients with preeclampsia (PE), fetal growth restriction (FGR), and normal controls.
Matched pairs analysis of third trimester placentae specimens (mean gestational age 36+2) was performed by semiquantitative measurements of the immunohistochemical staining intensities for progranulin and HIF-1α expression (PE n=13, FGR n=9 and controls n=11). Further, placental progranulin mRNA expression was analyzed by qRT-PCR on term placentae (n=3 for each group).
Compared to controls, villous trophoblast revealed a significantly higher expression of progranulin in cases of PE (P<0.05) and FGR (P<0.01). Similar results were shown for HIF-1α expression (P<0.01 for PE and <0.05 for FGR). The increase of the progranulin protein was not accompanied by an increase of the progranulin mRNA in term placentae.
Increased expression of progranulin protein in villous trophoblast cells in cases of PE and FGR may result from disturbed placental development and, therefore, may be of pathogenetic importance. The increase was correlated to HIF-1α expression. Further evaluation of this potential mechanism of regulation is required.
比较子痫前期（PE）、胎儿生长受限（FGR）和正常对照组胎盘绒毛滋养细胞中抗炎糖蛋白颗粒蛋白前体（progranulin）和缺氧诱导转录因子 1α（HIF-1α）的表达。
通过对第 3 孕期胎盘标本（平均孕龄 36+2）进行半定量免疫组织化学染色强度分析，对颗粒蛋白前体和 HIF-1α表达进行配对分析（PE 组 n=13，FGR 组 n=9，对照组 n=11）。进一步对足月胎盘（每组 3 例）进行 qRT-PCR 分析胎盘颗粒蛋白前体 mRNA 表达。
与对照组相比，PE（P<0.05）和 FGR（P<0.01）病例绒毛滋养细胞中颗粒蛋白前体表达显著升高。HIF-1α表达也显示出相似的结果（PE 组 P<0.01，FGR 组 P<0.05）。足月胎盘中颗粒蛋白前体蛋白的增加并不伴有颗粒蛋白前体 mRNA 的增加。
PE 和 FGR 绒毛滋养细胞中颗粒蛋白前体蛋白表达增加可能是由于胎盘发育异常所致，因此可能具有发病学意义。这种增加与 HIF-1α表达相关。需要进一步评估这种潜在的调节机制。
