Tumor chemosensitivity is correlated with expression of multidrug resistance associated factors in variously differentiated gastric carcinoma tissues.

BACKGROUND/AIMS: To evaluate the relationship between chemosensitivities in vitro and expressions of multidrug resistance (MDR) associated factors in differentiated gastric carcinomas.

METHODOLOGY

Gastric carcinomas tissues of varying degree of differentiation (65 cases) were collected and chemosensitivities to 5-FU, HCPT, PTX, L-OHP, CDDP and eADM were detected by sulphorhodamine B (SRB) assay, and expressions of P-gp, GST-π, Topo IIα, p53, Bcl-2, Bax and Survivin were tested by immunohistochemical staining.

RESULTS

Inhibition rates of 5-FU, L-OHP and CDDP for well differentiated tumors were lower than those of poorly differentiated tumors (p<0.05). Expressions of P-gp, Bcl-2 and Survivin were higher in well differentiated than in poorly differentiated carcinomas (p<0.05); while expression of Topo IIα in well differentiated carcinomas was lower than in poorly differentiated carcinomas (p<0.05). The expression of MDR factors was different between well and poorly differentiated carcinomas.

CONCLUSIONS

Different MDR characteristics were exhibited in well and poorly differentiated gastric carcinomas, which may be caused by different expressed MDR associated factors in these tissues.