Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Via D. Montesano 49, I-80131 Napoli, Italy.
Chembiochem. 2012 Oct 15;13(15):2219-24. doi: 10.1002/cbic.201200481. Epub 2012 Sep 4.
Several anti-HIV aptamers adopt DNA quadruplex structures. Among these, "Hotoda's aptamer" (base sequence TGGGAG) was one of the first to be discovered. Although it has been the topic of some recent research, no detailed structural investigations have been reported. Here we report structural investigations on this aptamer and analogues with related sequences, by using UV, CD, and NMR spectroscopy as well as electrophoretic techniques. The addition of a 3'-end thymine has allowed us to obtain a single, investigable quadruplex structure. Data clearly point to the presence of an A-tetrad. Furthermore, the effects of the incorporation of an 8-methyl-2'-deoxyguanosine at the 5'-end of the G-run were investigated.
几种抗 HIV 适体采用 DNA 四链体结构。其中，“Hotoda 的适体”（碱基序列为 TGGGAG）是最早被发现的适体之一。尽管它是最近一些研究的主题，但尚未报道详细的结构研究。在这里，我们通过使用紫外、圆二色性和 NMR 光谱以及电泳技术，报告了对该适体及其具有相关序列的类似物的结构研究。在 3' 端添加一个胸腺嘧啶，使我们能够获得一个单一的、可研究的四链体结构。数据清楚地表明存在一个 A-四联体。此外，还研究了在 G 链的 5' 端掺入 8-甲基-2'-脱氧鸟苷对结构的影响。
