Lehto K, Bubrick P, Dawson W O
Department of Plant Pathology, University of California, Riverside 92521.
Virology. 1990 Jan;174(1):290-3. doi: 10.1016/0042-6822(90)90077-5.
The kinetics of accumulation of the 30K and coat proteins of tobacco mosaic virus (TMV) which are expressed via subgenomic mRNAs were determined in near-synchronously infected cells of intact tobacco leaves. The 30K protein accumulated to maximal levels during the early stages of the infection as previously reported in tobacco protoplasts. However, in intact leaves, 30K protein accumulated for longer periods and attained higher concentrations than in protoplasts, remaining detectable in all later time samples. Detection of the coat protein, and also the 126K and 183K proteins, began at approximately the same time as the 30K protein (9-12 hr after initiation of replication), but their accumulation continued throughout the 72-hr sampling period. Most of the 30K protein accumulated before 24 hr, whereas most of the coat protein accumulated after 24 hr. This confirms that these two genes are regulated differently, both temporally and quantitatively.
在完整烟草叶片近乎同步感染的细胞中，测定了通过亚基因组mRNA表达的烟草花叶病毒（TMV）30K蛋白和外壳蛋白的积累动力学。如先前在烟草原生质体中报道的那样，30K蛋白在感染早期积累到最大水平。然而，在完整叶片中，30K蛋白积累的时间更长，浓度也比原生质体中的更高，在所有后续时间样本中均能检测到。外壳蛋白以及126K和183K蛋白的检测大约与30K蛋白同时开始（复制开始后9 - 12小时），但它们的积累在整个72小时的采样期内持续进行。大部分30K蛋白在24小时之前积累，而大部分外壳蛋白在24小时之后积累。这证实了这两个基因在时间和数量上的调控方式不同。
