Christen L, Seto J, Niles E G
Department of Biochemistry, State University of New York, Buffalo 14214.
Virology. 1990 Jan;174(1):35-42. doi: 10.1016/0042-6822(90)90051-r.
Vaccinia virus-infected BSC 40 cells do not permit the replication of superinfecting vaccinia virus. The extent of superinfecting virus propagation depends on the time of superinfection; there is 90% exclusion by 4 hr after the initial infection, and more than 99% by 6 hr. When superinfection is attempted at 6 hr after infection, the superinfecting virus is incapable of carrying out DNA replication or early gene transcription, demonstrating that an early event in the virus life cycle is inhibited. The rate of adsorption of the superinfecting virus is unaltered which shows that exclusion is affected at a point between adsorption and early gene transcription. In order to exclude superinfection, the primary infecting virus does not require replication of its DNA or expression of its late genes but it must express one or more early genes.
牛痘病毒感染的BSC 40细胞不允许超感染的牛痘病毒复制。超感染病毒的传播程度取决于超感染的时间；初次感染后4小时有90%的排斥率，6小时后超过99%。当在感染后6小时尝试超感染时，超感染病毒无法进行DNA复制或早期基因转录，这表明病毒生命周期中的一个早期事件受到抑制。超感染病毒的吸附速率未改变，这表明排斥在吸附和早期基因转录之间的某个点受到影响。为了排除超感染，初次感染的病毒不需要其DNA复制或晚期基因表达，但它必须表达一个或多个早期基因。
