Department of Chemistry, University of Victoria, Victoria, British Columbia V8P 5C2, Canada.
J Am Chem Soc. 2012 Sep 26;134(38):15937-45. doi: 10.1021/ja306596m. Epub 2012 Sep 18.
The synthesis and membrane activity of a suite of linear oligoesters containing a common diphenylacetylene unit core and differing in the hydroxyl terminus are reported. Active compounds formed high-conductance channels efficiently in both vesicle and planar bilayers, with one compound showing a very unusual slow loss of transport activity over a 20-30 min period. Steady-state and time-resolved fluorescence studies establish the rapid partition of active compounds to the bilayer and identify at least three types of membrane-associated species by their differing fluorescence lifetimes. The change in the distribution of species is correlated with the slow loss of activity. The results are interpreted in terms of an aggregate within a single bilayer leaflet that is nonetheless competent to transport ionic species through the bilayer. The properties of such structures, revealed by these compounds, appear to be consistent with commonly observed behaviors of other synthetic ion channels.
报道了一系列含有一个共同的二苯乙炔单元核心、羟基末端不同的线性低聚酯的合成和膜活性。活性化合物在囊泡和平面双层中都能有效地形成高电导通道，其中一种化合物在 20-30 分钟的时间内表现出非常异常的缓慢运输活性丧失。稳态和时间分辨荧光研究确定了活性化合物快速分配到双层，并通过其不同的荧光寿命鉴定了至少三种类型的膜相关物种。物种分布的变化与活性的缓慢丧失相关。结果根据单层叶片中的聚集物来解释，尽管如此，聚集物仍然有能力通过双层运输离子物种。这些化合物所揭示的此类结构的性质似乎与其他合成离子通道的常见行为一致。
