Balikesir University, Science and Art Faculty Department of Chemistry, Balikesir, Turkey.
Artif Cells Nanomed Biotechnol. 2013 Apr;41(2):125-30. doi: 10.3109/10731199.2012.696065. Epub 2012 Sep 4.
Paraoxonase (PON) was purified and characterized from the Merino and Kivircik sheep's blood serums by a two-step procedure using ammonium sulphate precipitation and Sepharose-4B-L-tyrosine-1-napthylamine hydrophobic interaction chromatography for the first time. On SDS-polyacyrilamide gel electrophoresis, purified human serum paraoxonase yielded a single band of 66 kDa on SDS-PAGE. The KM and Vmax were 0.482 mM and 41.348 U/mL.dak for Merino PON enzyme, 0.153 mM and 70.289 U/mL.dak for Kivircik PON, respectively. The effect of Mn(2+) , Hg(2+) , Co(2+) , Cd(2+) , Ni(2+) and Cu(2+) heavy metals on purified Merino and Kivircik serum PON in vitro was determined.
首次通过两步程序（硫酸铵沉淀和 Sepharose-4B-L-酪氨酸-1-萘胺疏水性相互作用色谱法）从美利奴羊和金维克里克羊的血清中纯化和表征了对氧磷酶（PON）。在 SDS-聚丙烯酰胺凝胶电泳上，纯化的人血清对氧磷酶在 SDS-PAGE 上产生 66 kDa 的单一条带。KM 和 Vmax 分别为 0.482 mM 和 41.348 U/mL.dak 用于美利奴 PON 酶，0.153 mM 和 70.289 U/mL.dak 用于金维克里克 PON。体外测定了 Mn（2+）、Hg（2+）、Co（2+）、Cd（2+）、Ni（2+）和 Cu（2+）重金属对纯化的美利奴和金维克里克血清 PON 的影响。
