Ontario Laboratory Network, Canadian Food Inspection Agency, Central Experimental Farm, Ottawa, Canada.
J Food Prot. 2012 Sep;75(9):1691-7. doi: 10.4315/0362-028X.JFP-12-116.
A cloth-based hybridization array system (CHAS) was developed for the identification of foodborne colony isolates of seven priority enterohemorrhagic Escherichia coli (EHEC-7) serogroups targeted by U. S. food inspection programs. Gene sequences associated with intimin; Shiga-like toxins 1 and 2; and the antigenic markers O26, O45, O103, O111, O121, O145, and O157 were amplified in a multiplex PCR incorporating a digoxigenin label, and detected by hybridization of the PCR products with an array of specific oligonucleotide probes immobilized on a polyester cloth support, with subsequent immunoenzymatic assay of the captured amplicons. The EHEC-7 CHAS exhibited 100 % inclusivity and 100 % exclusivity characteristics with respect to detection of the various markers among 89 different E. coli strains, with various marker gene profiles and 15 different strains of non-E. coli bacteria.
一种基于织物的杂交阵列系统 (CHAS) 被开发用于鉴定美国食品检测计划针对的七种优先肠出血性大肠杆菌 (EHEC-7) 血清群的食源性菌落分离株。与紧密素相关的基因序列;志贺样毒素 1 和 2;以及抗原标记物 O26、O45、O103、O111、O121、O145 和 O157 在包含地高辛标记的多重 PCR 中扩增，并通过与聚酯布支持物上固定的特定寡核苷酸探针阵列杂交来检测，随后对捕获的扩增子进行免疫酶分析。EHEC-7 CHAS 在检测 89 种不同大肠杆菌菌株中的各种标记物时表现出 100%包容性和 100%排他性特征，具有不同的标记基因谱和 15 种不同的非大肠杆菌细菌。
