人工甜味剂乙酰磺胺酸钾作为甜味受体激动剂对小肠细胞系葡萄糖摄取的影响。
Effect of the artificial sweetener, acesulfame potassium, a sweet taste receptor agonist, on glucose uptake in small intestinal cell lines.
机构信息
Department of Surgery and the Gastroenterology Research Unit, Mayo Clinic (GU 10-01), 200 1st Street SW, Rochester, MN 55905, USA.
出版信息
J Gastrointest Surg. 2013 Jan;17(1):153-8; discussion p. 158. doi: 10.1007/s11605-012-1998-z. Epub 2012 Sep 5.
INTRODUCTION
Sweet taste receptors may enhance glucose absorption.
AIM
This study aimed to explore the cell biology of sweet taste receptors on glucose uptake.
HYPOTHESIS
Artificial sweeteners increase glucose uptake via activating sweet taste receptors in the enterocyte to translocate GLUT2 to the apical membrane through the PLC βII pathway.
METHODS
Caco-2, RIE-1, and IEC-6 cells, starved from glucose for 1 h were pre-incubated with 10 mM acesulfame potassium (AceK). Glucose uptake was measured by incubating cells for 1 to 10 min with 0.5-50 mM glucose with or without U-73122, chelerythrine, and cytochalasin B.
RESULTS
In Caco-2 and RIE-1 cells, 10 mM AceK increased glucose uptake by 20-30 % at glucose >25 mM, but not in lesser glucose concentrations (<10 mM), nor at 1 min or 10 min incubations. U-73122 (PLC βII inhibitor) inhibited uptake at glucose >25 mM and for 5 min incubation; chelerythrine and cytochalasin B had similar effects. No effect occurred in IEC-6 cells. Activation of sweet taste receptors had no effect on glucose uptake in low (<25 mM) glucose concentrations but increased uptake at greater concentrations (>25 mM).
CONCLUSIONS
Role of artificial sweeteners on glucose uptake appears to act in part by effects on the enterocyte itself.
简介
甜味受体可能会增强葡萄糖吸收。
目的
本研究旨在探索甜味受体在葡萄糖摄取中的细胞生物学作用。
假说
人工甜味剂通过激活肠上皮细胞中的甜味受体，通过 PLC βII 途径将 GLUT2 转位到顶膜，从而增加葡萄糖摄取。
方法
将禁食 1 小时的 Caco-2、RIE-1 和 IEC-6 细胞用 10 mM 乙酰磺胺酸钾（AceK）预孵育。通过在有或没有 U-73122、Chelerythrine 和细胞松弛素 B 的情况下，用 0.5-50 mM 葡萄糖孵育细胞 1 至 10 分钟来测量葡萄糖摄取。
结果
在 Caco-2 和 RIE-1 细胞中，10 mM AceK 在葡萄糖浓度>25 mM 时可使葡萄糖摄取增加 20-30%，但在较低的葡萄糖浓度（<10 mM）或在 1 分钟或 10 分钟孵育时则不会。U-73122（PLC βII 抑制剂）在葡萄糖浓度>25 mM 和 5 分钟孵育时抑制摄取；Chelerythrine 和细胞松弛素 B 也有类似的作用。在 IEC-6 细胞中则没有作用。甜味受体的激活对低（<25 mM）葡萄糖浓度下的葡萄糖摄取没有影响，但在较高浓度（>25 mM）下增加了摄取。
结论
人工甜味剂对葡萄糖摄取的作用似乎部分是通过对肠上皮细胞本身的作用来实现的。
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