Department of Basic Sciences, Loma Linda University and Medical Center, Loma Linda, CA 92354, USA.
In Vivo. 2012 Sep-Oct;26(5):743-58.
BACKGROUND/AIM: To evaluate the impact of an antibiotic, minocycline, on several immune parameters in response to radiation in a mouse model.
C57BL/6 mice were treated with minocycline (i.p.) for 5 days, beginning immediately before radiation with 1-3 Gy (60)Co γ-rays. Spleen and blood were collected on day 4 post-irradiation. Cell populations were determined in the blood and spleen. Splenocytes were activated with anti-CD3 antibody for 48 h and cytokines were quantified.
Minocycline increased the counts and/or percentages of splenic macrophages, granulocytes, natural killer, T- and CD8(+) T-cells (p<0.05 versus radiation alone). Minocycline significantly increased the expression of interleukin-1α and β, which are radioprotective, as well as the ones of granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor, which accelerate neutrophil recovery (p<0.05 versus radiation alone), while suppressing cytokines that could prevent hematopoiesis, e.g. macrophage inflammatory protein-1α, tumor necrosis factor-α and interferon-γ.
These data indicate that minocycline should be further tested for use in restoration of the hematopoietic system after radiation exposure.
背景/目的：评估抗生素米诺环素对辐射后小鼠模型中几种免疫参数的影响。
C57BL/6 小鼠用米诺环素（腹腔注射）处理 5 天，在 1-3 Gy（60）Co γ射线照射前立即开始。在照射后第 4 天采集脾脏和血液。在血液和脾脏中确定细胞群体。用抗 CD3 抗体激活脾细胞 48 小时，并定量细胞因子。
米诺环素增加了脾脏巨噬细胞、粒细胞、自然杀伤细胞、T 细胞和 CD8(+)T 细胞的计数和/或百分比（与单独辐射相比，p<0.05）。米诺环素显著增加了白细胞介素-1α 和 β 的表达，这两种细胞因子具有放射保护作用，以及粒细胞-巨噬细胞集落刺激因子和粒细胞集落刺激因子的表达，这两种细胞因子加速了中性粒细胞的恢复（与单独辐射相比，p<0.05），同时抑制了可能阻止造血的细胞因子，例如巨噬细胞炎性蛋白-1α、肿瘤坏死因子-α 和干扰素-γ。
这些数据表明，米诺环素应进一步测试用于辐射暴露后造血系统的恢复。
