Bioorganic Chemistry Laboratory, Department of Chemistry, Inha University, Yunghyun-dong, Nam-gu, Incheon, 402-751, Republic of Korea.
Org Lett. 2012 Sep 21;14(18):4746-9. doi: 10.1021/ol301991h. Epub 2012 Sep 5.
A pyrene derivative chemosensor (Pyr-WH) based on a dipeptide shows a highly sensitive ratiometric response to Ag(I) as well as silver nanoparticles in aqueous solution at physiological pH. Pyr-WH penetrated live HeLa cells and exhibits a ratiometric response to intracellular Ag(I). The binding mode of Pyr-WH with Ag(I) was characterized based on fluorescence changes in different pH, NMR, and ESI mass spectrometer experiments.
基于二肽的芘衍生物化学传感器（Pyr-WH）在生理 pH 值的水溶液中对 Ag（I）以及银纳米粒子表现出高灵敏度的比率响应。Pyr-WH 穿透活 HeLa 细胞，并对细胞内 Ag（I）表现出比率响应。基于不同 pH 值、NMR 和 ESI 质谱实验中的荧光变化，对 Pyr-WH 与 Ag（I）的结合模式进行了表征。
