Department of Urology, Inonu University, Medical Faculty, Malatya, Turkey.
Ren Fail. 2012;34(9):1144-9. doi: 10.3109/0886022X.2012.717490. Epub 2012 Sep 6.
To investigate the protective effect of infliximab on ischemia-reperfusion (I/R) injury of the rat kidney.
Twenty-eight male Wistar albino rats were divided into four groups: sham-operated, I/R, I/R with infliximab administered before ischemia [I/R + infliximab (bi)], and I/R with infliximab administered before reperfusion [I/R + infliximab (br)]. After a right nephrectomy to produce damage, the left renal vessels were occluded for 60 min, followed by 24-h reperfusion in rats. Changes in the rat kidney were observed by measuring the tissue levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), and superoxide dismutase (SOD) and by evaluating hematoxylin-eosin (H&E)-stained and periodic acid-Schiff (PAS) sections.
The MDA and MPO levels in the I/R group were significantly higher than in the other groups (p < 0.05), and the SOD and GSH levels in the I/R + infliximab (bi) and I/R + infliximab (br) groups were significantly higher than in the I/R group (p < 0.05). However, histological examination revealed that the I/R + infliximab (bi) group and the I/R + infliximab (br) group had significantly fewer tubular changes and interstitial inflammatory cell infiltration than the I/R group.
These results show that infliximab may protect against I/R injury in the rat I/R model.
研究英夫利昔单抗对大鼠肾缺血再灌注（I/R）损伤的保护作用。
28 只雄性 Wistar 白化大鼠随机分为 4 组：假手术组、I/R 组、缺血前给予英夫利昔单抗的 I/R 组（I/R+英夫利昔单抗预处理组，bi）和再灌注前给予英夫利昔单抗的 I/R 组（I/R+英夫利昔单抗预处理组，br）。在右肾切除造成损伤后，结扎左肾血管 60 分钟，然后再灌注 24 小时。通过测量组织丙二醛（MDA）、髓过氧化物酶（MPO）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）的水平，以及苏木精-伊红（H&E）染色和过碘酸-Schiff（PAS）染色切片，观察大鼠肾脏的变化。
与其他组相比，I/R 组的 MDA 和 MPO 水平显著升高（p<0.05），而 I/R+英夫利昔单抗预处理组（bi）和 I/R+英夫利昔单抗预处理组（br）的 SOD 和 GSH 水平显著升高（p<0.05）。然而，组织学检查显示，I/R+英夫利昔单抗预处理组（bi）和 I/R+英夫利昔单抗预处理组（br）的肾小管变化和间质炎症细胞浸润明显少于 I/R 组。
这些结果表明，英夫利昔单抗可能对大鼠 I/R 模型中的 I/R 损伤具有保护作用。
