电解氧化水对黄曲霉毒素 B1 的脱毒作用及其产物鉴定与安全性评价。

Product identification and safety evaluation of aflatoxin B1 decontaminated by electrolyzed oxidizing water.

机构信息

College of Food Science and Nutritional Engineering, China Agricultural University , P.O. Box 40, No. 17 Qing hua dong lu, Hai dian, Beijing 100083, People's Republic of China.

出版信息

J Agric Food Chem. 2012 Sep 26;60(38):9770-8. doi: 10.1021/jf303478y. Epub 2012 Sep 14.

DOI:10.1021/jf303478y

PMID:22950859

Abstract

In this study with aflatoxin-contaminated peanuts, the effectiveness of electrolyzed oxidizing water (EOW) in the decontamination of aflatoxin B(1) was investigated. The aflatoxin B(1) content was markedly reduced upon treatment with EOW, particularly with neutral electrolyzed oxidizing water (NEW). The conversion product of EOW treatment was isolated and identified as 8-chloro-9-hydroxy aflatoxin B(1) (compound 1), which is an amphiphilic molecule, in contrast to fat-soluble aflatoxin B(1). A mutagenic response study revealed that the number of revertants per plate after treatment of bacterial strains TA-97, TA-98, TA-100, and TA-102 with NEW was within the standard value range. The HepG2 cell viability assay yielded an IC(50) value of compound 1 approximately 150 mM. This study indicates that EOW had the ability to decontaminate aflatoxin B(1), and the conversion product, compound 1, did not exhibit mutagenic activity or cytotoxic effects.

摘要

本研究采用黄曲霉毒素污染花生作为材料，考察了次氯酸水（EOW）对黄曲霉毒素 B1（AFB1）的脱毒效果。结果表明，EOW，尤其是中性次氯酸水（NEW）处理可显著降低 AFB1 含量。EOW 处理的转化产物被分离鉴定为 8-氯-9-羟基黄曲霉毒素 B1（化合物 1），与脂溶性的 AFB1 不同，该化合物为两亲分子。致突变性研究结果显示，用 NEW 处理 TA-97、TA-98、TA-100 和 TA-102 菌株后，每平板的回复突变数均在标准值范围内。HepG2 细胞活力测定得出化合物 1 的 IC50 值约为 150 mM。本研究表明 EOW 具有脱毒 AFB1 的能力，且转化产物化合物 1 不具有致突变性或细胞毒性。