Suzuki Tetsuya, Noro Takashi, Kawamura Yukio, Fukunaga Kenji, Watanabe Masumi, Ohta Mari, Sugiue Hisashi, Sato Yuri, Kohno Masahiro, Hotta Kunimoto
Laboratory of Food Wholesomeness and Soundness, Division of Life Sciences, Graduate School of Fisheries Science, Hokkaido University, Minato, Hakodate 041-8611, Japan.
J Agric Food Chem. 2002 Jan 30;50(3):633-41. doi: 10.1021/jf0108361.
Electrolysis of a 0.1% (17.1 mM) solution of NaCl using separate anode and cathode compartments gives rise to solutions containing active chemical species. The strongly acidic "anode solution" (EW+) has high levels of dissolved oxygen and available chlorine in a form of hypochlorous acid (HOCl) with a strong potential for sterilization, which we have investigated here. Exposing Aspergillus parasiticus at an initial density of 10(3)spores in 10 microL to a 50-fold volume (500 microL) of EW+ containing ca. 390 micromol HOCl for 15 min at room temperature resulted in a complete inhibition of fungal growth, whereas the cathode solution (EW-) had negligible inhibitory effects. Moreover, the mutagenicity of aflatoxin B(1) (AFB(1)) for Salmonella typhimurium TA-98 and TA-100 strains was strongly reduced after AFB(1) exposure to the EW+ but not with the EW-. In high-performance liquid chromatography analysis, the peak corresponding to AFB(1) disappeared after treatment with the EW+, indicating decomposition of the aflatoxin. In contrast, the routinely used disinfectant sodium hypochlorite, NaOCl, of the same available chlorine content as that of EW+ but in a different chemical form, hypochlorite (OCl-) ion, did not decompose AFB(1) at pH 11. However, NaOCl did decompose AFB(1) at pH 3, which indicated that the principle chemical formula to participate in the decomposition of AFB(1) is not the OCl- ion but HOCl. Furthermore, because the decomposition of AFB(1) was suppressed by pretreating the EW+ with the OH radical scavenger thiourea, the chemical species responsible for the AFB(1)-decomposing property of the EW+ should be at least due to the OH radical originated from HOCl. The OH in EW+ was proved by electron spin resonance analysis.
使用单独的阳极室和阴极室对0.1%（17.1 mM）的氯化钠溶液进行电解，会产生含有活性化学物质的溶液。强酸性的“阳极溶液”（EW+）含有高浓度的溶解氧和以次氯酸（HOCl）形式存在的有效氯，具有很强的杀菌潜力，我们在此对其进行了研究。将初始密度为10³个孢子的寄生曲霉在10微升中暴露于50倍体积（500微升）含有约390微摩尔HOCl的EW+中，在室温下放置15分钟，导致真菌生长完全受到抑制，而阴极溶液（EW-）的抑制作用可忽略不计。此外，黄曲霉毒素B₁（AFB₁）对鼠伤寒沙门氏菌TA-98和TA-100菌株的致突变性在AFB₁暴露于EW+后大幅降低，但暴露于EW-后则没有变化。在高效液相色谱分析中，与AFB₁对应的峰在经EW+处理后消失，表明黄曲霉毒素发生了分解。相比之下，常规使用的消毒剂次氯酸钠（NaOCl），其有效氯含量与EW+相同，但化学形式为次氯酸根（OCl⁻）离子，在pH值为11时不会分解AFB₁。然而，NaOCl在pH值为3时确实会分解AFB₁，这表明参与AFB₁分解的主要化学式不是OCl⁻离子，而是HOCl。此外，由于用羟基自由基清除剂硫脲对EW+进行预处理会抑制AFB₁的分解，因此EW+具有分解AFB₁特性的化学物质至少应归因于源自HOCl的羟基自由基。通过电子自旋共振分析证明了EW+中存在羟基自由基。
