Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
Peptides. 2012 Nov;38(1):41-53. doi: 10.1016/j.peptides.2012.08.008. Epub 2012 Aug 19.
We have cloned, synthesized, and characterized 11 novel antimicrobial peptides from a skin derived cDNA library of the Chungan torrent frog, Amolops chunganensis. Seven of the 11 antimicrobial peptides were present in authentic A. chunganensis skin secretions. Sequence analysis indicated that the 11 peptides belonged to the temporin, esculentin-2, palustrin-2, brevinin-1, and brevinin-2 families. The peptides displayed potent antimicrobial activities against several strains of microorganisms. One peptide, brevinin-1CG5, demonstrated antimicrobial activity against all tested Gram-positive and Gram-negative bacteria and fungi, and showed high antimicrobial potency (MIC=0.6 μM) against Gram-positive bacterium Rhodococcus rhodochrous. Some peptides also demonstrated weak hemolytic activity against human erythrocytes in vitro. Phylogenetic analysis based on the amino acid sequences of brevinin-1, brevinin-2, and esculentin-2 peptides from family Ranidae confirmed that the current taxonomic status of A. chunganensis is correct.
我们从中国大鲵(Chungan torrent frog,Amolops chunganensis)的皮肤 cDNA 文库中克隆、合成并鉴定了 11 种新型抗菌肽。这 11 种抗菌肽中有 7 种存在于真正的 A. chunganensis 皮肤分泌物中。序列分析表明,这 11 种肽属于蛙皮素、海葵素-2、沼泽蛙素-2、brevienin-1 和 brevinin-2 家族。这些肽对几种微生物菌株表现出强大的抗菌活性。一种肽,brevienin-1CG5,对所有测试的革兰氏阳性和革兰氏阴性细菌和真菌均具有抗菌活性,对革兰氏阳性菌 Rhodococcus rhodochrous 具有很高的抗菌效力(MIC=0.6 μM)。一些肽在体外对人红细胞也表现出较弱的溶血活性。基于 Ranidae 家族的 brevinin-1、brevienin-2 和 esculentin-2 肽的氨基酸序列进行的系统发育分析证实,A. chunganensis 的当前分类地位是正确的。