Dipartimento Farmaco Chimico Tecnologico, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy.
Food Chem. 2012 Dec 1;135(3):1985-90. doi: 10.1016/j.foodchem.2012.06.096. Epub 2012 Jul 4.
Riboflavin (vitamin B(2)) and its metabolite lumichrome were quantified in 117 samples from 11 unifloral honeys types (Arbutus unedo L., Asphodelus microcarpus Salzm. et Viv., Citrus spp., Eucalyptus spp., Hedysarum coronarium L., Castanea sativa L. honeydew, Mentha spp., Paliurus spina-christi., Salix spp., Salvia officinalis L., Satureja spp.). The quantification of these two compounds was performed by LC-DAD method which does not require sample purification. The proposed method in our study has low limits of detection and quantification, very good linearity in a large concentration range and very good precision. It allows simultaneous determination of 5-hydroxymethylfurfural (HMF) and known chemical biomarkers of unifloral honeys such as abscisic acid diastereomers, homogentisic acid, methyl syringate and kynurenic acid. No statistical correlation was observed between riboflavin and lumichrome content. Although, the concentration of vitamin B(2) in honey may be too low (<6.1mg/kg) to generate interest in the field of nutrition, the presence of its main metabolite lumichrome may be useful to determine the botanical origin of certain unifloral honeys. In fact, the analysis of 11 unifloral honey types showed that Dalmatian sage (S. officinalis L.) honey is characterised by unusual high levels of lumichrome (20.2±2.6mg/kg). The botanical origin of lumichrome from sage flower was assessed by analysing bee-stomach extracts. Other analytical parameters, such as total phenols, antioxidant and antiradical activities, HMF and diastase activity were studied in Dalmatian sage honey.
核黄素（维生素 B(2)）及其代谢产物光黄素在 117 个样本中进行了定量分析，这些样本来自 11 种单花蜜类型（杨梅、小丛薰衣草、柑橘属、桉树属、黄花补血草、板栗蜜露、荆豆蜜、柳树、鼠尾草、迷迭香）。这两种化合物的定量采用 LC-DAD 方法进行，该方法不需要样品纯化。本研究提出的方法具有较低的检测限和定量限，在较大浓度范围内具有非常好的线性度和良好的精密度。它允许同时测定 5-羟甲基糠醛（HMF）和单花蜜的已知化学标志物，如脱落酸差向异构体、反式-对羟基肉桂酸、甲基丁香酸和犬尿氨酸。未观察到核黄素和光黄素含量之间存在统计学相关性。尽管蜂蜜中维生素 B(2)的浓度可能过低（<6.1mg/kg），不足以引起营养领域的兴趣，但主要代谢产物光黄素的存在可能有助于确定某些单花蜜的植物来源。实际上，对 11 种单花蜜类型的分析表明，达尔马提亚鼠尾草（S. officinalis L.）蜂蜜的光黄素含量异常高（20.2±2.6mg/kg）。通过分析蜜蜂胃提取物评估了鼠尾草花中光黄素的植物来源。还研究了其他分析参数，如总酚、抗氧化和抗自由基活性、HMF 和酶活性在达尔马提亚鼠尾草蜂蜜中的作用。
