Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Freie Universität of Berlin, Berlin, Germany.
Clin Hemorheol Microcirc. 2012;52(2-4):67-84. doi: 10.3233/CH-2012-1585.
Intense angiogenesis, vascular remodelling as well as regression of its vasculature are prerequisites for ovarian function with its cyclically developing and regressing follicles and corpora lutea. So far neither a stringent explanation for the enormous angiogenic potential of the ovary nor its cellular origins have been suggested. In an earlier study of our work group, endothelial cells were isolated from the bovine corpus luteum and cultivated in vitro. They performed vasulogenesis in vitro and showed properties of progenitor cells. The present study aimed at in situ identification of endothelial progenitor cells (EPCs) in the bovine ovary. Immunohistochemical examinations, based on the detection of KDR and CD34 co-labelled cells - a marker combination that amongst others is commonly accepted as typical for EPC identification - were performed. Hormonal cycle dependent expression varieties were analysed by the measurement of mRNA amounts of CD34 and KDR as well as the stem cell marker CD133 (Prominin-1). Ovarian samples comprising corpora lutea of varying stages (developing and mature corpus luteum, corpus luteum in regression, corpus luteum of pregnancy) from 17 adult cows were examined. Results show that specific mRNA of CD133, CD34 and KDR was expressed in ovaries of all luteal stages. Expression data analysis revealed significant differences in CD133 and CD34 expression levels between the luteal stages but no significant differences in KDR expression. CD34/KDR co-immunoreactive cells were predominantly situated within the media of arterial vessel wall. The detection of ovarian EPCs represents an important step towards further understanding of the mechanisms involved in the reproductive biology and pathophysiology of the ovary.
强烈的血管生成、血管重塑以及其血管的退化是卵巢功能的先决条件，其周期性地发育和退化卵泡和黄体。到目前为止，对于卵巢巨大的血管生成潜力及其细胞起源，既没有严格的解释，也没有提出。在我们工作组的早期研究中，我们从牛黄体中分离出内皮细胞并在体外培养。它们在体外进行血管生成，并表现出祖细胞的特性。本研究旨在原位鉴定牛卵巢中的内皮祖细胞(EPCs)。基于检测 KDR 和 CD34 共标记细胞的免疫组织化学检查——这是一种被广泛接受的鉴定 EPC 的典型标志物组合——进行了检查。通过测量 CD34 和 KDR 的 mRNA 量以及干细胞标志物 CD133（Prominin-1），分析了激素周期依赖性表达变化。检查了来自 17 头成年奶牛的卵巢样本，包括不同阶段（发育和成熟黄体、退化黄体、妊娠黄体）的黄体。结果表明，所有黄体阶段的卵巢都表达了特定的 CD133、CD34 和 KDR mRNA。表达数据分析显示，CD133 和 CD34 表达水平在黄体阶段之间存在显著差异，但 KDR 表达水平没有显著差异。CD34/KDR 共免疫反应性细胞主要位于动脉血管壁的中膜。卵巢 EPC 的检测是进一步了解卵巢生殖生物学和病理生理学相关机制的重要步骤。
