Discipline of Immunology, Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, São Paulo, Brazil.
Immunobiology. 2013 Apr;218(4):609-19. doi: 10.1016/j.imbio.2012.07.032. Epub 2012 Aug 7.
B-1 cells can be differentiated from B-2 cells because they are predominantly located in the peritoneal and pleural cavities and have distinct phenotypic patterns and activation properties. The role of both cell populations in cancer progression is still controversial. Previous studies have indicated that direct contact between B-1 cells and B16 melanoma tumor cells (B16) increases the metastatic potential of the tumor cells. However, cellular changes that are induced in B-1 cells during the interaction between these two cell types have not been evaluated. In the present study, it is hypothesized that B-1 cells are modified after their interaction with tumor cells, leading to both increased cell viability and rate of proliferation. Additionally, soluble factors that were secreted by B16 cells were sufficient to augment B-1 cell viability and to modify the production of IL-10 by B-1 cells. Impressively, after direct or indirect contact with the B16 cells, B-1 cells became resistant to radiation-induced cell death. Thus, future studies that assess the importance of concomitant immunity and other conventional therapies in cancer treatment are needed.
B-1 细胞可以从 B-2 细胞中分化出来，因为它们主要位于腹腔和胸腔中，具有独特的表型模式和激活特性。这两种细胞群体在癌症进展中的作用仍存在争议。先前的研究表明，B-1 细胞与 B16 黑色素瘤肿瘤细胞（B16）之间的直接接触会增加肿瘤细胞的转移潜能。然而，在这两种细胞类型相互作用过程中诱导的 B-1 细胞的细胞变化尚未得到评估。在本研究中，假设 B-1 细胞在与肿瘤细胞相互作用后发生了改变，导致细胞活力和增殖率均增加。此外，B16 细胞分泌的可溶性因子足以增强 B-1 细胞活力并改变 B-1 细胞产生 IL-10 的能力。令人印象深刻的是，B-1 细胞在与 B16 细胞直接或间接接触后，对辐射诱导的细胞死亡产生了抗性。因此，未来需要评估伴随免疫和其他常规疗法在癌症治疗中的重要性的研究。
