由出芽短梗霉(Aureobasidium pullulans)利用农业生物质底物生产聚(β-L-苹果酸)(PMA)。
Production of poly(β-L-malic acid) (PMA) from agricultural biomass substrates by Aureobasidium pullulans.
机构信息
Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
出版信息
Biotechnol Lett. 2013 Jan;35(1):83-9. doi: 10.1007/s10529-012-1045-x. Epub 2012 Sep 7.
For the first time the production of poly(β-L -malic acid) (PMA) has been achieved using agricultural biomass substrates by the yeast-like fungus Aureobasidium pullulans. Strains NRRL Y-2311-1, NRRL 50382, NRRL 50383, and NRRL 50384, representing diverse isolation sources and phylogenetic clades, produced PMA from alkaline H(2)O(2)-pretreated corn fiber and wheat straw as sole carbon sources. Pretreated wheat straw was better than pretreated corn fiber, and strain NRRL 50383 gave the highest overall yields of PMA. The addition of CaCO(3) plus supplementary hydrolytic enzymes enhanced PMA production. Four basal media were compared for PMA production, and the best was found to be a N-limited pullulan production medium (PM). In this medium, PMA production took place during growth limitation. Under optimal conditions, strain NRRL 50383 produced more than 20 g PMA/l from 5 % (w/v) pretreated wheat straw in PM with 3 % (w/v) CaCO(3) and supplementary enzymes.
首次利用酵母样真菌出芽短梗霉以农业生物质为底物生产聚（β-L-苹果酸）（PMA）。代表不同分离源和系统发育分支的菌株 NRRL Y-2311-1、NRRL 50382、NRRL 50383 和 NRRL 50384 从碱性 H₂O₂预处理的玉米纤维和小麦秸秆作为唯一碳源生产 PMA。预处理的小麦秸秆优于预处理的玉米纤维，菌株 NRRL 50383 给出了最高的 PMA 总产量。添加 CaCO₃加补充水解酶可提高 PMA 产量。比较了四种基础培养基用于 PMA 生产，发现最佳的是一种 N 限制出芽短梗霉生产培养基（PM）。在该培养基中，在生长受限的情况下发生 PMA 生产。在最佳条件下，菌株 NRRL 50383 在 PM 中用 3%（w/v）CaCO₃和补充酶从 5%（w/v）预处理的小麦秸秆中生产超过 20 g PMA/L。
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