Kim Jong Shik, Lee In Kyoung, Yun Bong Sik
Gyeongbuk Institute for Marine Bioindustry, 22 Haeyanggwahak-gil, Uljin, Gyeongbuk 767-813, Republic of Korea.
Division of Biotechnology and Advanced Institute of Environmental and Bioscience, Chonbuk National University, 79 Gobong-ro, Iksan, Chonbuk 570-752, Republic of Korea.
PLoS One. 2015 Apr 7;10(4):e0122917. doi: 10.1371/journal.pone.0122917. eCollection 2015.
Yeast biosurfactants are important biotechnological products in the food industry, and they have medical and cosmeceutical applications owing to their specific modes of action, low toxicity, and applicability. Thus, we have isolated and examined biosurfactant-producing yeast for various industrial and medical applications. A rapid and simple method was developed to screen biosurfactant-producing yeasts for high production of eco-friendly biosurfactants. Using this method, several potential niches of biosurfactant-producing yeasts, such as wild flowers, were investigated. We successfully selected a yeast strain, L3-GPY, with potent surfactant activity from a tiger lily, Lilium lancifolium Thunb. Here, we report the first identification of strain L3-GPY as the black yeast Aureobasidium pullulans. In addition, we isolated a new low-surface-tension chemical, designated glycerol-liamocin, from the culture supernatant of strain L3-GPY through consecutive chromatography steps, involving an ODS column, solvent partition, silica gel, Sephadex LH-20, and an ODS Sep-Pak cartridge column. The chemical structure of glycerol-liamocin, determined by mass spectrometry and nuclear magnetic resonance spectroscopy, indicates that it is a novel compound with the molecular formula C33H62O12. Furthermore, glycerol-liamocin exhibited potent biosurfactant activity (31 mN/m). These results suggest that glycerol-liamocin is a potential novel biosurfactantfor use in various industrial applications.
酵母生物表面活性剂是食品工业中重要的生物技术产品，由于其特定的作用方式、低毒性和适用性，它们在医学和药妆领域也有应用。因此，我们分离并研究了用于各种工业和医学应用的产生物表面活性剂酵母。我们开发了一种快速简便的方法来筛选高产环保型生物表面活性剂的产生物表面活性剂酵母。利用这种方法，我们研究了产生物表面活性剂酵母的几个潜在来源，如野花。我们成功地从卷丹（Lilium lancifolium Thunb.）中筛选出了具有强大表面活性剂活性的酵母菌株L3-GPY。在此，我们首次鉴定出菌株L3-GPY为黑酵母出芽短梗霉（Aureobasidium pullulans）。此外，我们通过连续的色谱步骤，包括ODS柱、溶剂分配、硅胶、Sephadex LH-20和ODS Sep-Pak柱，从菌株L3-GPY的培养上清液中分离出一种新的低表面张力化合物，命名为甘油-利亚莫星。通过质谱和核磁共振光谱确定的甘油-利亚莫星的化学结构表明，它是一种分子式为C33H62O12的新型化合物。此外，甘油-利亚莫星表现出强大的生物表面活性剂活性（31 mN/m）。这些结果表明，甘油-利亚莫星是一种可用于各种工业应用的潜在新型生物表面活性剂。
