Jelicks L A, Gupta R K
Department of Physiology, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York 10461.
J Biol Chem. 1990 Jan 25;265(3):1394-400.
We have utilized multinuclear NMR spectroscopy to examine the relationship between cytosolic free Ca2+ ([Ca2+]in), free Mg2+ ([Mg2+]in) and intracellular Na+ ([Na+]in) levels of the intact thoracic aorta and primary hypertension using the Wistar-Kyoto and Sprague-Dawley rats as controls and the spontaneously hypertensive rat as a model for genetic hypertension. Cytosolic free [Ca2+] was measured using 19F NMR of the intracellular Ca2+ indicator 5,5'-difluoro-1,2-bis-(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, free [Mg2+] using the 31P resonances of intracellular ATP, and intracellular [Na+] by 23Na NMR in combination with the extracellular shift reagent dysprosium tripolyphosphate. We have found that both the [Na+]in and [Ca2+]in levels were significantly increased in the hypertensive animals relative to normotensive controls (p less than 0.01). Mean systolic blood pressures (using tail cuff method) of control and hypertensive rats were 123 +/- 8 mm Hg (mean +/- 2 S.E., n = 7) and 159 +/- 6 mm Hg (mean +/- 2 S.E., n = 7), respectively. [Na+]in and [Ca2+]in were 21.9 +/- 6.4 mM (mean +/- 2 S.E., n = 7) and 277 +/- 28 nM (mean +/- 2 S.E., n = 5) for the spontaneously hypertensive rats versus 10.1 +/- 1.8 mM (mean +/- 2 S.E., n = 7) and 151 +/- 26 nM (mean +/- 2 S.E., n = 5) for control rats, respectively. A slight difference observed between intracellular free Mg2+ levels in hypertensives (180 +/- 38 microM, mean +/- 2 S.E., n = 4) and controls (246 +/- 76 microM, mean +/- 2 S.E., n = 4) was not statistically significant (p greater than 0.1). These data indicate alterations in the cell membrane ion transport function of the aortic smooth muscle in primary hypertension.
我们利用多核核磁共振波谱技术，以Wistar-Kyoto大鼠和Sprague-Dawley大鼠作为对照，自发性高血压大鼠作为遗传性高血压模型，研究了完整胸主动脉及原发性高血压中细胞溶质游离钙离子（[Ca2+]in）、游离镁离子（[Mg2+]in）和细胞内钠离子（[Na+]in）水平之间的关系。使用细胞内钙离子指示剂5,5'-二氟-1,2-双（邻氨基苯氧基）乙烷-N,N,N',N'-四乙酸的19F核磁共振测量细胞溶质游离[Ca2+]，利用细胞内ATP的31P共振测量游离[Mg2+]，并结合细胞外位移试剂三聚磷酸镝通过23Na核磁共振测量细胞内[Na+]。我们发现，与血压正常的对照组相比，高血压动物的[Na+]in和[Ca2+]in水平均显著升高（p<0.01）。对照大鼠和高血压大鼠的平均收缩压（采用尾袖法）分别为123±8 mmHg（平均值±2标准误，n = 7）和159±6 mmHg（平均值±2标准误，n = 7）。自发性高血压大鼠的[Na+]in和[Ca2+]in分别为21.9±6.4 mM（平均值±2标准误，n = 7）和277±28 nM（平均值±2标准误，n = 5），而对照大鼠分别为10.1±1.8 mM（平均值±2标准误，n = 7）和151±26 nM（平均值±2标准误，n = 5）。高血压患者（180±38 μM，平均值±2标准误，n = 4）和对照组（246±76 μM，平均值±2标准误，n = 4）细胞内游离镁离子水平的轻微差异无统计学意义（p>0.1）。这些数据表明原发性高血压中主动脉平滑肌细胞膜离子转运功能发生了改变。
