Integrated Department of Immunology, National Jewish Health and University of Colorado School of Medicine, Denver, CO 80206, USA.
Immunol Res. 2013 Mar;55(1-3):146-54. doi: 10.1007/s12026-012-8358-7.
Multi-photon microscopy has taken hold as a widely used technique in immunology, allowing for imaging of the kinetics of immune cell motility and cell-cell interactions, but what have we learned from this technique about the processes involved in peripheral tolerance and autoimmunity? Various studies have now looked at the dynamics of several mechanisms of peripheral T cell tolerance and efforts to examine the dynamics of the autoimmune response at the disease site are also under way. Here, we will discuss the findings of studies that use multi-photon microscopy to examine the dynamics of T cell tolerance in the lymph nodes and of the autoimmune processes involved in models of type 1 diabetes and multiple sclerosis. An emerging theme from these studies is that short T cell-antigen presenting cell interactions can lead to tolerance, and that autoreactive T cell restimulation at the disease site can play an important role in autoimmune disease exacerbation.
多光子显微镜已成为免疫学界广泛应用的技术，可用于观察免疫细胞运动和细胞间相互作用的动力学，但我们从该技术中学到了哪些关于外周耐受和自身免疫过程的知识呢？目前已有多项研究着眼于外周 T 细胞耐受的几种机制的动力学，并且正在努力研究疾病部位自身免疫反应的动力学。在这里，我们将讨论使用多光子显微镜检查淋巴结中 T 细胞耐受动力学以及 1 型糖尿病和多发性硬化症模型中涉及的自身免疫过程的研究结果。这些研究的一个新主题是，短时间的 T 细胞-抗原呈递细胞相互作用可导致耐受，而疾病部位的自身反应性 T 细胞再刺激可能在自身免疫病恶化中起重要作用。
