雪花莲（雪滴花）凝集素的结晶及初步X射线衍射结果
Crystallization and preliminary X-ray diffraction results of snowdrop (Galanthus nivalis) lectin.
作者信息
Wright C S, Kaku H, Goldstein I J
机构信息
Department of Biochemistry and Molecular Biophysics, MCV/VCU, MCV Station, Richmond 23298-0001.
出版信息
J Biol Chem. 1990 Jan 25;265(3):1676-7.
Well-ordered single crystals have been grown for a mannose-specific lectin from snowdrop (Galanthus nivalis) bulbs in the presence of methyl-alpha-D-mannoside. The space group symmetry is consistent with the orthorhombic space group P2(1)2(1)2(1). The unit cell parameters are a = 140.0 A, b = 64.7 A, c = 62.0 A. The asymmetric unit can accommodate a tetramer. The functional molecule (50,000 daltons) consists of four identical subunits and is highly specific for alpha 1,3-linked mannose oligosaccharides.
在α-D-甲基甘露糖苷存在的情况下，已从雪花莲（Galanthus nivalis）鳞茎中培养出了有序的甘露糖特异性凝集素单晶。空间群对称性与正交空间群P2(1)2(1)2(1)一致。晶胞参数为a = 140.0 Å，b = 64.7 Å，c = 62.0 Å。不对称单元可容纳一个四聚体。功能分子（50,000道尔顿）由四个相同的亚基组成，对α1,3-连接的甘露糖寡糖具有高度特异性。
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