Cátedra de Genética, Universidad de Buenos Aires. Av. San Martin 4453, Buenos Aires, Argentina.
J Insect Sci. 2011;11:174. doi: 10.1673/031.011.17401.
The bloodsucking horn fly, Haematobia irritans (L.) (Diptera: Muscidae), is one of the most damaging pests of pasture cattle in many areas of the world. Both male and female imagoes spend their adult stage on the host, while immature stages develop in dung. Our goal was to determine if the progress of H. irritans gonad maturation can be correlated with eye and cuticle pigmentation events that occur during development of the imago within the puparium. The progression of germline cell divisions in immature gonads was analyzed from the beginning of the third larval instar (48 hours after egg hatch) until imago ecdysis. In the developing male larval gonad, meiosis began 72 hours after egg hatch, whereas in females oogonia were premeiotic at 72 hours. Meiosis was not detected in females until the mid-pharate adult stage, 120 hours after puparium formation. Therefore, gonad maturation in females appears to be delayed 144 hours with respect to that in males. In the stages within the puparium, the timing of germline cell division events was correlated with the progress of pigmentation of the eyes and cuticle as external markers.
吸血虻，Haematobia irritans（L.）（双翅目：蝇科），是世界许多地区牧场牛群最具破坏性的害虫之一。雌雄成虫都在宿主身上度过成虫阶段，而未成熟阶段则在粪便中发育。我们的目标是确定吸血虻性腺成熟的进展是否可以与成虫在蛹内发育过程中发生的眼睛和表皮色素沉着事件相关联。从第三龄幼虫开始（孵化后 48 小时）到成虫蜕皮，分析了未成熟性腺中生殖细胞分裂的进展。在发育中的雄性幼虫性腺中，减数分裂在孵化后 72 小时开始，而在雌性中，卵原细胞在 72 小时前处于减数分裂前。在形成蛹后 120 小时的成虫中期阶段，才在雌性中检测到减数分裂。因此，与雄性相比，雌性的性腺成熟似乎延迟了 144 小时。在蛹内的各个阶段，生殖细胞分裂事件的时间与眼睛和表皮色素沉着作为外部标记的进展相关。
