一种用于构建适合不育释放计划的转基因昆虫品系的可抑制的雌性特异性致死遗传系统。
A repressible female-specific lethal genetic system for making transgenic insect strains suitable for a sterile-release program.
作者信息
Heinrich J C, Scott M J
机构信息
Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand.
出版信息
Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8229-32. doi: 10.1073/pnas.140142697.
Abstract
We have developed a tetracycline-repressible female-specific lethal genetic system in the vinegar fly Drosophila melanogaster. One component of the system is the tetracycline-controlled transactivator gene under the control of the fat body and female-specific transcription enhancer from the yolk protein 1 gene. The other component consists of the proapoptotic gene hid under the control of a tetracycline-responsive element. Males and females of a strain carrying both components are viable on medium supplemented with tetracycline, but only males survive on normal medium. A strain with such properties would be ideal for a sterile-insect release program, which is most effective when only males are released in the field.
摘要
我们在黑腹果蝇中开发了一种四环素可抑制的雌性特异性致死遗传系统。该系统的一个组件是四环素控制的反式激活因子基因，其受脂肪体和来自卵黄蛋白1基因的雌性特异性转录增强子的控制。另一个组件由受四环素反应元件控制的促凋亡基因hid组成。携带这两个组件的品系的雄性和雌性在添加了四环素的培养基上可以存活，但只有雄性在正常培养基上存活。具有这种特性的品系对于不育昆虫释放计划来说将是理想的，当仅在田间释放雄性时该计划最为有效。
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