CIISA, Technical University of Lisbon, Lisbon, Portugal.
J Reprod Immunol. 2012 Dec;96(1-2):45-57. doi: 10.1016/j.jri.2012.07.003. Epub 2012 Sep 5.
The objectives of this study were to evaluate the following events in the canine endometrium over the course of the oestrous cycle: (i) the transcriptional profiles of genes encoding the Toll-like receptors (TLR1-TLR7 and TLR9); (ii) the transcription and protein expression levels of TLR2 and TLR4; (iii) the gene transcription profile of prostaglandin synthesis enzymes (PTGS2, PGES and PGFS); (iv) the response pattern of PGF(2α) and PGE(2) following exposure of endometrial explants to LPS and LTA. TLR1-TLR7 and TLR9 genes were transcribed in the endometrium of bitches throughout the oestrous cycle, which indicates that TLR-mediated immune surveillance is an important component of the defence mechanisms within the uterus. Canine endometrial mRNA and protein expression of TLR2 and TLR4 was up-regulated at the late dioestrus and anoestrus and was the lowest in the follicular phase and early dioestrus. The decreased mRNA and protein levels observed at early dioestrus may favour implantation, but may also be linked to the high prevalence of pyometra at this stage of the oestrous cycle. After LPS and LTA stimulation, endometrial explants produced more PGF(2α) than PGE(2), which may be related to the early demise of the corpus luteum observed in vivo in canine pyometra cases. Overall, these results indicate that TLRs are involved in the activation of the inflammatory response associated with pyometra in the bitch. TLRs may therefore be therapeutic targets for the control of uterine bacterial infections in the bitch and potentially in other species.
（i）编码 Toll 样受体（TLR1-TLR7 和 TLR9）的基因的转录谱；（ii）TLR2 和 TLR4 的转录和蛋白表达水平；（iii）前列腺素合成酶（PTGS2、PGES 和 PGFS）的基因转录谱；（iv）子宫内膜外植体暴露于 LPS 和 LTA 后 PGF(2α)和 PGE(2)的反应模式。TLR1-TLR7 和 TLR9 基因在发情周期中的母犬子宫内膜中均有转录，这表明 TLR 介导的免疫监视是子宫内防御机制的重要组成部分。犬子宫内膜 TLR2 和 TLR4 的 mRNA 和蛋白表达在发情后期和乏情期上调，并在卵泡期和发情早期最低。发情早期观察到的 mRNA 和蛋白水平降低可能有利于着床，但也可能与发情周期此阶段高发子宫蓄脓有关。在 LPS 和 LTA 刺激后，子宫内膜外植体产生的 PGF(2α)多于 PGE(2)，这可能与犬子宫蓄脓病例中体内观察到的黄体早期死亡有关。总的来说，这些结果表明 TLR 参与了与犬子宫蓄脓相关的炎症反应的激活。因此，TLR 可能是控制犬和其他物种子宫细菌感染的治疗靶点。
