Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário-Trindade - 88040-900, Florianópolis-SC, Brazil.
Pharmacol Biochem Behav. 2012 Dec;103(2):220-9. doi: 10.1016/j.pbb.2012.08.024. Epub 2012 Aug 31.
The olfactory bulbectomy (OB) is an animal model of depression that results in behavioral, neurochemical and neuroendocrinological changes, features comparable to those seen in depressive patients. This study investigated OB-induced alterations in locomotor activity and exploratory behavior in the open-field test, self-care and motivational behavior in the splash test, hyperactivity in the novel object test and novel cage test, and the influence of chronic treatment with fluoxetine (10mg/kg, p.o., once daily for 14days) on these parameters. Fluoxetine reversed OB-induced hyperactivity in the open-field test, locomotor hyperactivity and the increase in exploratory behavior induced by novelty in the novel object and novel cage tests, and the loss of self-care and motivational behavior in the splash test. Moreover, OB decreased the number of grooming and fecal boli in the open-field and novel cage tests, alterations that were not reversed by fluoxetine. OB caused an increase in hippocampal, but not in prefrontal acetylcholinesterase (AChE) activity. Fluoxetine was able to reverse the increase in hippocampal AChE activity induced by OB. Serum corticosterone was increased in SHAM and bulbectomized mice treated with fluoxetine. In conclusion, OB mice exhibited depressive-like behaviors associated with an increase in hippocampal AChE activity, effects that were reversed by chronic treatment with fluoxetine.
嗅球切除术（OB）是一种抑郁症的动物模型，它会导致行为、神经化学和神经内分泌变化，具有与抑郁症患者相似的特征。本研究探讨了 OB 诱导的旷场试验中运动活性和探索行为、飞溅试验中自我护理和动机行为、新奇物体试验和新奇笼试验中过度活跃以及慢性氟西汀（10mg/kg，p.o.，每日一次，共 14 天）治疗对这些参数的影响。氟西汀逆转了 OB 诱导的旷场试验中过度活跃、新奇物体和新奇笼试验中运动活性增加和探索行为增加，以及飞溅试验中自我护理和动机行为的丧失。此外，OB 减少了开放场和新奇笼试验中的梳理和粪便球数量，氟西汀未能逆转这些变化。OB 增加了海马体但不增加前额叶乙酰胆碱酯酶（AChE）活性。氟西汀能够逆转 OB 诱导的海马体 AChE 活性增加。SHAM 和用氟西汀治疗的嗅球切除术小鼠的血清皮质酮增加。总之，OB 小鼠表现出与海马体 AChE 活性增加相关的抑郁样行为，这些行为可通过慢性氟西汀治疗逆转。
