Department of Biochemistry, Center of Biological Sciences, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis, SC, Brazil.
J Ethnopharmacol. 2012 Aug 30;143(1):158-69. doi: 10.1016/j.jep.2012.06.017. Epub 2012 Jun 18.
Rosemary, Rosmarinus officinalis L., has several therapeutic applications in folk medicine for the treatment of a wide range of diseases, including depression.
To evaluate the ability of Rosmarinus officinalis hydroalcoholic extract (ROHE), as compared to the positive control fluoxetine, to reverse behavioral (hyperactivity, anhedonic behavior and learning deficit in water maze) and biochemical alterations (serum glucose level and acetylcholinesterase, AChE, activity) induced by an animal model of depression, the olfactory bulbectomy (OB) in mice.
Locomotor and exploratory behavior was assessed in the open-field, novel object and novel cage tests, anhedonic behavior was assessed in the splash test; cognitive deficits were evaluated in the water maze task. For the first set of experiments, ROHE (10-300 mg/kg) or fluoxetine (10mg/kg) was administered once daily (p.o.) for 14 days after OB and the behavioral tests were performed. For the second set of experiments, serum glucose and hippocampal and cerebrocortical AChE activity were determined in OB and SHAM-operated mice treated orally with ROHE (10mg/kg), fluoxetine (10mg/kg) or vehicle.
ROHE (10-300 mg/kg), similar to fluoxetine, reversed OB-induced hyperactivity, increased exploratory and anhedonic behavior. OB needed significantly more trials in the training session to acquire the spatial information, but they displayed a similar profile to that of SHAM mice in the test session (24h later), demonstrating a selective deficit in spatial learning, which was not reversed by ROHE or fluoxetine. A reduced serum glucose level and an increased hippocampal AChE activity were observed in bulbectomized mice; only the latter effect was reversed by fluoxetine, while both effects were reversed by ROHE.
ROHE exerted an antidepressant-like effect in bulbectomized mice and was able to abolish AchE alterations and hypoglycemia, but not spatial learning deficit induced by OB. Overall, results suggest the potential of Rosmarinus officinalis for the treatment of depression, validating the traditional use of this plant.
迷迭香，迷迭香 officinalis L.，在民间医学中有多种治疗应用，可治疗多种疾病，包括抑郁症。
评估迷迭香 officinalis 水醇提取物 (ROHE) 的能力，与阳性对照氟西汀相比，可逆转动物抑郁症模型（嗅球切除术 [OB] 在小鼠中）引起的行为（多动、快感缺失行为和水迷宫学习缺陷）和生化改变（血清葡萄糖水平和乙酰胆碱酯酶，AChE，活性）。
在旷场、新物体和新笼测试中评估运动和探索行为，在飞溅测试中评估快感缺失行为；在水迷宫任务中评估认知缺陷。对于第一组实验，ROHE（10-300mg/kg）或氟西汀（10mg/kg）在 OB 后每天口服（po.）给药一次，共 14 天，然后进行行为测试。对于第二组实验，在口服 ROHE（10mg/kg）、氟西汀（10mg/kg）或载体的 OB 和 SHAM 手术小鼠中测定血清葡萄糖和海马和大脑皮质 AChE 活性。
ROHE（10-300mg/kg）与氟西汀相似，可逆转 OB 诱导的多动、增加的探索和快感缺失行为。OB 在训练阶段需要更多的试验来获得空间信息，但它们在测试阶段（24 小时后）表现出与 SHAM 小鼠相似的模式，表明空间学习存在选择性缺陷，而 ROHE 或氟西汀并未逆转。在球囊切除的小鼠中观察到血清葡萄糖水平降低和海马 AChE 活性增加；只有后者的作用被氟西汀逆转，而两者的作用都被 ROHE 逆转。
ROHE 在 OB 切除的小鼠中表现出抗抑郁作用，并能够消除 AchE 改变和低血糖，但不能逆转 OB 引起的空间学习缺陷。总体而言，结果表明迷迭香 officinalis 治疗抑郁症的潜力，验证了这种植物的传统用途。
