N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
Carbohydr Res. 2012 Nov 1;361:27-32. doi: 10.1016/j.carres.2012.07.021. Epub 2012 Aug 8.
Mild acid degradation of the lipopolysaccharide (LPS) of Providencia alcalifaciens O3 followed by GPC on Sephadex G-50 and anion-exchange chromatography on DEAE-Trisacryl M afforded neutral and acidic polysaccharides, LPS core oligosaccharide, and an oligosaccharide composed of one repeat of the neutral polysaccharide (O-unit) linked to the LPS core. The following structure of the pentasaccharide O-unit was established by sugar and methylation analyses, 2D (1)H and (13)C NMR spectroscopy and ESI MS: [formula: see text] where Qui3NFo stands for 3,6-dideoxy-3-formamidoglucose and GalAN for galacturonamide. The LPS core is represented by the Glc(3)Gal(1)GalA(1)Hep(3)Kdo(1)Ara4N(1)P(3)EtN(2) glycoform reported earlier for P. alcalifaciens O9, O34, and O19. The acidic polysaccharide had the same peptidoglycan-like structure as a polysaccharide isolated earlier from P. alcalifaciens O24, O38, and O45, and, most likely, represents bacterial capsule material.
温和酸降解产碱普罗威登斯菌 O3 的脂多糖 (LPS)，然后通过 GPC 在 Sephadex G-50 上和阴离子交换层析在 DEAE-Trisacryl M 上进行，得到中性和酸性多糖、LPS 核心寡糖和由一个重复中性多糖 (O-单元) 与 LPS 核心连接而成的寡糖。通过糖和甲基化分析、2D (1)H 和 (13)C NMR 光谱和 ESI MS 确定了五糖 O-单元的以下结构：[公式：见文本]，其中 Qui3NFo 代表 3,6-二脱氧-3-甲酰胺葡萄糖，GalAN 代表半乳糖醛酰胺。LPS 核心由 Glc(3)Gal(1)GalA(1)Hep(3)Kdo(1)Ara4N(1)P(3)EtN(2)糖型表示，此前已报道产碱普罗威登斯菌 O9、O34 和 O19 中存在这种糖型。酸性多糖具有与早先从产碱普罗威登斯菌 O24、O38 和 O45 中分离出的多糖相同的肽聚糖样结构，很可能代表细菌荚膜物质。
