Biochemistry Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. dr sanaa
Dis Markers. 2013;34(1):57-62. doi: 10.3233/DMA-2012-0923.
In a trial to improve the diagnostic efficacy of conventional urine cytology we determine survivin RNA and matrix metalloproteinase 2 and 9 in urine of bladder cancer cases.
Voided urine specimens were collected from patients with histologically confirmed bladder urothelial carcinoma (Group 1; n=46), urological patients without urothelial carcinoma (Group 2; n=20), and healthy volunteers (Group 3; n=20). Urine cytology, survivin RNA was estimated by qualitative nested RT-PCR and MMP-2, MMP-9 activity were detected by gelatin zymography. The expression of survivin RNA and matrix metalloproteinase 2 and 9 in bladder cancer was compared with benign and normal cases.
Positivity rates of survivin RNA and MMPs zymography were significantly different among the 3 groups. Urine survivin detection by qualitative nested RT-PCR showed 76.1% sensitivity and 95% specificity. The overall sensitivity, specificity of urinary MMP zymography was 67.3%, 90% respectively. The combined use of urine cytology with urine survivin or MMPs zymography increased sensitivity of urine cytology from 50% to 84.7%. The highest sensitivity (95.6%) was obtained on combining the three markers.
Survivin RNA and MMPS zymography can be considered as promising noninvasive markers for bladder cancer early detection. Combined use of the three markers improved the sensitivity for detecting bladder cancer.
在一项旨在提高常规尿液细胞学诊断效能的试验中，我们测定了膀胱癌患者尿液中的生存素 RNA 以及基质金属蛋白酶 2 和 9。
收集经组织学证实的膀胱尿路上皮癌患者（第 1 组，n=46）、无尿路上皮癌的泌尿科患者（第 2 组，n=20）和健康志愿者（第 3 组，n=20）的晨尿标本。通过定性巢式 RT-PCR 测定尿液中的生存素 RNA，通过明胶酶谱法检测 MMP-2 和 MMP-9 的活性。将膀胱癌患者的生存素 RNA 和基质金属蛋白酶 2、9 的表达与良性和正常病例进行比较。
3 组之间生存素 RNA 和 MMPs 酶谱的阳性率差异有统计学意义。定性巢式 RT-PCR 检测尿液中的生存素显示出 76.1%的敏感性和 95%的特异性。尿 MMP 酶谱的总体敏感性和特异性分别为 67.3%和 90%。尿细胞学联合尿生存素或 MMPs 酶谱检测可将尿细胞学的敏感性从 50%提高到 84.7%。联合使用这 3 种标志物可获得最高的敏感性（95.6%）。
生存素 RNA 和 MMPs 酶谱可作为膀胱癌早期检测有前途的非侵入性标志物。联合使用这 3 种标志物可提高膀胱癌的检测敏感性。
