Department of Anatomy, Akita University Graduate School of Medicine and Faculty of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan.
Neurosci Res. 2012 Oct;74(2):91-105. doi: 10.1016/j.neures.2012.08.005. Epub 2012 Aug 31.
ATP-sensitive K(+) (K(ATP)) channel subunits SUR2A and SUR2B in the rat brain were investigated by RT-PCR assay, western blot analysis, in situ hybridization histochemistry, and immunohistochemical staining. The results show that the mRNA and protein of SUR2A and SUR2B are expressed in whole rat brain extracts and selected regions. SUR2 mRNA is widely expressed in many neurons and glial cells as revealed by in situ hybridization histochemistry. Immunohistochemical staining shows SUR2A to be widely expressed in neurons of the brain, especially in the large pyramidal neurons and their main dendrites in the neocortex and in the Purkinje cells of the cerebellar cortex. In contrast to SUR2A, SUR2B is potently expressed in small cells in the corpus callosum and cerebellar white matter, but is also weakly expressed in some neurons. Double immunostaining shows SUR2B to be localized in astrocytes and oligodendrocytes, while SUR2A is only localized in oligodendrocytes. These results suggest that SUR2A might be mainly a regulatory subunit of the K(ATP) channel in most neurons and part of oligodendrocytes, while SUR2B might be mainly a regulatory subunit of the K(ATP) channel in astrocytes, oligodendrocytes, and some neurons.
采用 RT-PCR 检测、Western blot 分析、原位杂交组织化学和免疫组织化学染色等方法研究了大鼠脑内 ATP 敏感性钾（KATP）通道亚单位 SUR2A 和 SUR2B。结果表明，SUR2A 和 SUR2B 的 mRNA 和蛋白均存在于全脑提取物和部分脑区。原位杂交组织化学显示 SUR2 广泛表达于多种神经元和神经胶质细胞。免疫组织化学染色显示 SUR2A 广泛表达于脑内神经元，尤其是新皮质的大锥体神经元及其主树突和小脑皮质的浦肯野细胞。与 SUR2A 相反，SUR2B 强烈表达于胼胝体和小脑白质的小细胞，但也弱表达于部分神经元。双免疫染色显示 SUR2B 定位于星形胶质细胞和少突胶质细胞，而 SUR2A 仅定位于少突胶质细胞。这些结果提示 SUR2A 可能主要作为大多数神经元和部分少突胶质细胞中 KATP 通道的调节亚单位，而 SUR2B 可能主要作为星形胶质细胞、少突胶质细胞和部分神经元中 KATP 通道的调节亚单位。
