Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Cochin University of Science and Technology, Cochin, 682 022, Kerala, India.
J Mater Sci Mater Med. 2012 Dec;23(12):2913-21. doi: 10.1007/s10856-012-4754-8. Epub 2012 Sep 8.
The development of nanoparticles containing active molecules having improved stability, sustained release and maximum half life helps in cell proliferation result in enhanced tissue regeneration. Our study focuses on the use of Gamma amino butyric acid (GABA) and serotonin (5-HT) coupled chitosan nanoparticles for the active liver regeneration in male Wistar rats. The nanoparticles were prepared and the morphology was studied using SEM. The FT-IR spectra of the nanoparticles and the maximum encapsulation efficiency of GABA and 5-HT binding to chitosan nanoparticles were observed. The in vitro release studies provided the percentage release of GABA and 5-HT from the nanoparticles at different time intervals. The quantification of DNA and protein syntheses was done using [(3)H] thymidine and [(3)H] leucine uptake studies that determined the enhancement in hepatocyte proliferation. Our results project the role of GABA and 5-HT chitosan nanoparticles in the treatment of liver based diseases.
载有活性分子的纳米粒子的发展提高了稳定性、缓释性和半衰期，有助于细胞增殖，从而促进组织再生。我们的研究集中在使用γ-氨基丁酸（GABA）和血清素（5-HT）偶联壳聚糖纳米粒子实现雄性 Wistar 大鼠的主动肝再生。制备了纳米粒子，并使用 SEM 研究了其形态。观察了纳米粒子的 FT-IR 光谱以及 GABA 和 5-HT 与壳聚糖纳米粒子结合的最大包封效率。体外释放研究提供了不同时间间隔从纳米粒子中释放 GABA 和 5-HT 的百分比。通过 [(3)H]胸苷和 [(3)H]亮氨酸摄取研究进行了 DNA 和蛋白质合成的定量，该研究确定了肝细胞增殖的增强。我们的研究结果表明 GABA 和 5-HT 壳聚糖纳米粒子在治疗肝脏疾病方面的作用。
