Department of Occupational Hygiene, Institute of Health and Environmental Medicine, Tianjin 300050, China.
J Membr Biol. 2013 Jan;246(1):7-11. doi: 10.1007/s00232-012-9495-6. Epub 2012 Sep 9.
We morphologically and physiologically characterized Madin-Darby canine kidney (MDCK) cell and mouse principal cell of kidney in cortical collecting duct (mpkCCD) via hopping probe ion conductance microscopy, transepithelial electrical resistance (TEER) measurements, and single-channel recordings. The specific membrane structures of microvilli and tight junctions were clearly observed in MDCK and mpkCCD cell monolayers. The electrophysiological functions of epithelial Na(+) channel in MDCK and mpkCCD cells were further characterized by measuring amiloride-sensitive TEER values for the whole-cell monolayer and detecting the ion channel activities with patch clamping. Our results provide more morphological and functional information to help better utilize these two mammalian CCD cell lines for mechanism studies of sodium absorption and reabsorption in the distal nephron.
我们通过跳跃探针离子电导显微镜、跨上皮电阻（TEER）测量和单通道记录对 Madin-Darby 犬肾（MDCK）细胞和小鼠肾皮质集合管主细胞（mpkCCD）进行了形态和生理学表征。MDCK 和 mpkCCD 细胞单层中清晰地观察到微绒毛和紧密连接的特定膜结构。通过测量全细胞单层对氨苯蝶啶敏感的 TEER 值以及用膜片钳检测离子通道活性，进一步表征了 MDCK 和 mpkCCD 细胞上皮钠离子通道的电生理功能。我们的研究结果提供了更多的形态和功能信息，有助于更好地利用这两种哺乳动物 CCD 细胞系来研究远曲小管中钠离子吸收和重吸收的机制。
