Zhou Yuan, Chen Xuewei, Liu Xiao, Lu Hujie, Li Ying, Zhu Hui, An Gaihong, Zhang Na, Zhang Jianning, Ma Qiang, Zhang Yanjun
Department of Neurosurgery, Tianjin Medical University General Hospital; Tianjin Neurological Institute; Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, 154 Anshan Road, Heping District, Tianjin, 300052, China.
J Membr Biol. 2014 Jun;247(6):461-8. doi: 10.1007/s00232-014-9647-y. Epub 2014 Apr 11.
Involvement of phosphoinositide 3-kinases (PI3Ks) in early aldosterone action on epithelial sodium channel (ENaC) in mammalian renal epithelia was investigated by hopping probe ion conductance microscopy combined with patch-clamping in this study. Aldosterone treatment enlarged the cell volume and elevated the apical membrane of renal mpkCCDc14 epithelia, which resulted in enhancing the open probability of ENaC. Inhibition of PI3K pathway by LY294002 obviously suppressed these aldosterone-induced changes in both cell morphology and ENaC activity. These results indicated the important role of PI3K pathway in early aldosterone action and the close relationship between cell morphology and ENaC activity in mammalian renal epithelia.
在本研究中，通过跳变探针离子电导显微镜结合膜片钳技术，研究了磷酸肌醇3激酶（PI3Ks）在醛固酮对哺乳动物肾上皮细胞上皮钠通道（ENaC）早期作用中的参与情况。醛固酮处理使肾mpkCCDc14上皮细胞体积增大，顶膜升高，这导致ENaC开放概率增加。LY294002对PI3K通路的抑制明显抑制了醛固酮诱导的细胞形态和ENaC活性的这些变化。这些结果表明PI3K通路在醛固酮早期作用中的重要作用以及哺乳动物肾上皮细胞中细胞形态与ENaC活性之间的密切关系。
